Development and validation of an UHPLC-HRMS protocol for the analysis of flavan-3-ol metabolites and catabolites in urine, plasma and feces of rats fed a red wine proanthocyanidin extract

Food Chemistry
Gema Pereira-CaroAlan Crozier

Abstract

This study developed, optimized and validated an ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) method to identify and quantify metabolites and microbial-derived catabolites in urine, plasma and feces of rats following ingestion of 50 mg of a red wine proanthocyanidin-rich extract. The method was validated for specificity, linearity, limit of detection (LD) and quantification (LQ), intra-day and inter-day precision, recovery and matrix effects, which were determined for 34 compounds in the three biological matrices. After method validation, three parent flavan-3-ols, four 5-carbon side chain ring fission metabolites, and 27 phenolic acid and aromatic catabolites were quantified in plasma, urine and feces after red wine proanthocyanidin intake. These results establish the value of the UHPLC-HRMS protocol in obtaining a detailed picture of proanthocyanidin metabolites and their microbial-derived catabolites, along with their phase II metabolites, in biological fluids of rat, and potentially in human clinical studies designed to evaluate the bioavailability of dietary flavan-3-ols.

Citations

Apr 30, 2020·Food & Function·Gema Pereira-CaroAlan Crozier
Jun 17, 2020·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Raúl González-DomínguezCristina Andrés-Lacueva
Jan 30, 2021·Food Research International·Mariem AchourRaquel Mateos
Jul 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Wenyang TaoShiguo Chen
Jun 12, 2021·Food Research International·Cristina Magdaleno-TapiaJara Pérez-Jiménez

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