Development and Validation of an Ultra-Sensitive UHPLC-MS/MS Method for Neonicotinoid Analysis in Milk

Journal of Agricultural and Food Chemistry
Laurence Lachat, G Glauser

Abstract

A very sensitive ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for the quantitation of the most common neonicotinoids (thiamethoxam, clothianidin, imidacloprid, acetamiprid, and thiacloprid) at trace levels in milk. Using fast and selective liquid-liquid extraction (LLE) starting from 0.5 mL of milk, lowest limits of quantitation (LLOQ) equal or lower than 10 pg/mL for all analytes were achieved. Precision and accuracy were evaluated at four different concentrations (5, 10, 500, and 10000 pg/mL) and ranged between 2 and 16% (RSD) and 77-125%, respectively. Extraction recoveries and matrix effects ranged between 64 and 76% and 88-98%, respectively. The method was applied to measure neonicotinoid levels in a series of conventional and organic Swiss milks as well as in human breast milk and commercial powdered milk. More than 90% of the samples tested positive for at least one neonicotinoid. However, all animal samples were far below the maximum residue limits authorized for human consumption with average total neonicotinoid levels of 16.1 ± 13.1 pg/mL. Human breast milks and powdered milks contained similar amounts of neonicotinoids. Taken together, our resul...Continue Reading

References

Jun 23, 2010·Journal of Agricultural and Food Chemistry·Peter JeschkeAlfred Elbert
Apr 28, 2011·Journal of Agricultural and Food Chemistry·Chris SackRobert E Smith
Oct 27, 2011·Journal of Agricultural and Food Chemistry·Gina Tanner, Christoph Czerwenka
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Jul 8, 2016·Environmental Health Perspectives·Andria M CiminoMelissa J Perry
Oct 7, 2017·Science·E A D MitchellA Aebi

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Citations

Aug 1, 2019·Journal of Biochemical and Molecular Toxicology·Marc Le VéeOlivier Fardel
Jan 27, 2019·Bulletin of Environmental Contamination and Toxicology·Giorgio FedrizziPaola Roncada
Jun 28, 2020·Communications Biology·Daniel SchläppiPeter Neumann

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