PMID: 18727534Aug 30, 2008Paper

Development and validation of column high-performance liquid chromatographic and derivative spectrophotometric methods for determination of levofloxacin and ornidazole in combined dosage forms

Journal of AOAC International
Satish A PatelJayesh B Vaghmasi

Abstract

The manuscript describes validated reversed-phase column high-performance liquid chromatographic (RP-HPLC) and first-derivative UV spectrophotometric methods for the estimation of levofloxacin (LFX) and ornidazole (ORNI) in combined dosage forms. The RP-HPLC separation was achieved on a Phenomenex C18 column (250 mm x 4.6 mm id, 5 microm) using KH2PO4 buffer (pH 6.8)-methanol-acetonitrile (70 + 15 + 15, v/v/v) mobile phase at a flow rate of 1.5 mL/min and ambient temperature (25 +/- 2 degrees C). Quantification was achieved with photodiode array detection at 295 nm over the concentration range of 1-10 microg/mL for both LFX and ORNI, with mean recovery of 101.7 +/- 0.23 and 99.23 +/- 1.57%, respectively, by the RP-HPLC method. The derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The first-order derivative spectra were obtained at N = 1 (scaling factor), Deltalambda = 2.0 nm (wavelength interval), and the determinations were made at 310 nm (ZCP of ORNI) for LFX and 295 nm (ZCP of LFX) for ORNI over the concentration range of 2-40 microg/mL for both LFX and ORNI. Mean recovery was 99.46 +/- 0.96 and 100.9 +/- 0.72%, respectively, by the first-deri...Continue Reading

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