Development and validation of isomer specific RP-HPLC method for quantification of cefpodoxime proxetil

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Vasu Kumar KakumanuArvind K Bansal

Abstract

The present work explains the development and validation of a simple and reliable isomer specific liquid chromatographic method for the quantitative determination of cefpodoxime proxetil (CP) in rat in situ intestinal perfusate samples. Chromatography was carried out by reversed-phase technique on a C-18 column with a mobile phase composed of 20 mM ammonium acetate buffer (pH 5.0) and acetonitrile in the ratio of 62:38 pumped at a flow-rate of 1 ml/min. The detection was carried out at 235 nm and a column temperature of 30 degrees C. The method was evaluated for the various validation parameters, such as linearity, accuracy, precision, LOD, LOQ, specificity, selectivity, and sample stability. The results of intra- and inter-day validation (n = 3) showed the method to be efficient and the same was applied in an in situ permeability study conducted for CP in rats.

References

Jun 17, 1994·Journal of Chromatography. B, Biomedical Applications·F CamusR Farinotti
Mar 4, 1994·Journal of Chromatography. B, Biomedical Applications·M J LovdahlD M Canafax

❮ Previous
Next ❯

Citations

Mar 4, 2008·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Vasu Kumar KakumanuArvind Kumar Bansal
Feb 26, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anil DubalaRenjith George
Aug 11, 2007·Journal of Pharmaceutical and Biomedical Analysis·Salwa R El-ShabouryAzza H Rageh
Dec 13, 2012·Magnetic Resonance in Chemistry : MRC·Shivani Mahajan, Inder Pal Singh
Jul 3, 2007·Advanced Drug Delivery Reviews·Jochem Alsenz, Manfred Kansy
Dec 7, 2018·Critical Reviews in Analytical Chemistry·Karen de Souza RuganiHérida Regina Nunes Salgado

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Pharmaceutics
Vasu Kumar KakumanuArvind K Bansal
European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
Vasu Kumar KakumanuArvind K Bansal
© 2021 Meta ULC. All rights reserved