PMID: 2497344May 1, 1989Paper

Development and validation of the spiral Salmonella assay: an automated approach to bacterial mutagenicity testing

Mutation Research
V S HoukL D Claxton

Abstract

Since its development by Dr. Bruce Ames and his colleagues more than a decade ago, the Salmonella/mammalian microsome mutagenicity assay has become a widely accepted tool to assist in the identification of chemicals with mutagenic and carcinogenic potential. Several automated approaches to Salmonella testing have been proposed in recent years but have failed to gain acceptance in the scientific community due to poor performance or lack of demonstrated usefulness. In this paper we report on an automated system that successfully generates dose-response data and, moreover, reduces the labor, materials, and sample mass required to obtain such information. In the standard plate-incorporation assay, dose-response relationships are defined by testing discrete doses of the test agent on a series of agar plates. In contrast, the spiral Salmonella assay generates dose-response data from a continuous concentration gradient on a single agar plate. Upon analysis, each spiral plate yields a dose-response curve consisting of 13 data points that span a concentration range of about 15:1, which is equivalent to 5 two-fold serial dilutions. The performance of the spiral Salmonella assay was compared to that of the conventional plate-incorporation...Continue Reading

Citations

Aug 1, 1996·Biodegradation·L E RuddL D Claxton
Jan 1, 1992·Mutation Research·T HamasakiH Kito
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·J V KosmoskiM J Smerdon
Aug 5, 2010·Environmental Health Perspectives·Larry D ClaxtonDavid M DeMarini
Oct 1, 1993·Environmental Health Perspectives·C S AaronD M Zimmer
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Antonio ConconiMichael J Smerdon
Dec 25, 2012·Mutation Research·P A EscobarB Mahadevan
Dec 2, 2004·Mutation Research·Larry D ClaxtonSarah H Warren
Jul 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Peng MaoJohn J Wyrick
Nov 30, 2004·Advances in Applied Microbiology·Kristien Mortelmans, Doppalapudi S Rupa
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Jun 5, 2003·The Journal of Biological Chemistry·Hee-Chul AhnBong-Jin Lee
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Jan 1, 1992·Environmental and Molecular Mutagenesis·V S HoukH Matsushita
Jan 1, 1991·Environmental and Molecular Mutagenesis·V S HoukL D Claxton
Oct 8, 2005·The Journal of Biological Chemistry·Zeljko M SvedruzićMichael J Smerdon

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