Development and validation of the spectrophotometric method of butaphosphan determination in veterinary preparations

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Mariia SmolinskaIhor Kotsiumbas

Abstract

A simple and sensitive method has been developed for the determination of butaphosphan in single- and multi-component veterinary preparations. The method is based on the combustion of organic matter prior to the formation of phosphate and the subsequent formation of the phosphoric‑molybdenum complex in the presence of molybdate and ascorbic acid as a reducing agent in the sulfate acid medium. The intensity of light absorption of the colored analytical form of "molybdenum blue" is measured at a wavelength of 830 nm in the range of 0.3-9.0 μg/ml of butaphosphan, where Beer's law is obeyed, has been measured. The reaction conditions and other experimental parameters influencing the reaction transition and the stability of the colored complex have been thoroughly investigated and optimized for the quantitative determination of butaphosphan. Validation of the developed method according to the requirements has been carried out State Pharmacopoeia of Ukraine garmonised with European Pharmacopoeia and its suitability for the analysis of the selected veterinary preparations for the quantitative content of butaphosfan has been confirmed. The results of the determination of butaphosphan in six veterinary preparations of domestic and impor...Continue Reading

References

Jan 11, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·T UedaK Shimizu
Oct 1, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Mohamed Nooreldeen Abbas

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