Development, characterization, and preliminary evaluation of a temperature-sensitive mutant of Ornithobacterium rhinotracheale for potential use as a live vaccine in turkeys

Avian Diseases
Vanessa LopesKakambi V Nagaraja

Abstract

A temperature-sensitive (Ts) mutant strain of Ornithobacterium rhinotracheale (ORT) was developed after exposure of the wild-type organism to N-methyl-N'-nitro-N-nitrosoguanidine. The Ts mutant strain grew at 31 C but had its growth inhibited at 41 C unlike wild-type parent strain. The Ts mutant and parent strains were characterized. Morphologic and biochemical properties of wild-type and mutant strains did not show any differences. The strains were also characterized by polymerase chain reaction (PCR)-based fingerprinting methods. Results showed similar patterns in repetitive sequences by repetitive PCR (enterobacterial repetitive intergenic consensus, highly conserved repeated DNA elements present in Streptococcus pneumoniae (BOX), repetitive extragenic palindromic, and Salmonella enteritidis repetitive element primers); however, random amplified polymorphic DNA fingerprinting was able to differentiate mutant and parent strains showing a unique pattern for each of the ORT strains. The rationale for the use of a Ts strain as a vaccine is based on the ability of the mutant to colonize the upper respiratory tract but not the lower respiratory tract and systemic system of the birds, where the wild-type strain causes severe lesion...Continue Reading

References

Aug 26, 1998·Poultry Science·J R Glisson
Jan 8, 1999·Journal of Medical Microbiology·G RajashekaraV Kapur
Aug 17, 1999·Journal of Clinical Microbiology·S AlamD Beighton
Nov 10, 2000·The Veterinary Record·L van VeenP van Empel
Jun 1, 1999·Avian Pathology : Journal of the W.V.P.A·P C van Empel, H M Hafez

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Citations

Nov 13, 2007·Avian Pathology : Journal of the W.V.P.A·T R Gopala Krishna MurthyR Kalaimathi
May 22, 2020·Bulletin of the National Research Centre·Nagwa S Rabie, Zeinab M S Amin Girh
Jan 2, 2020·Veterinary Sciences·Eunice Ventura BarbosaHelena Carla Castro

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Methods Mentioned

BETA
optical microscopy
PCR
enzyme-linked immunosorbent assay
ELISA

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