PMID: 11320664Apr 26, 2001Paper

Development, characterization and therapy of a disseminated model of childhood neuroblastoma in SCID mice

Cancer Chemotherapy and Pharmacology
J ThompsonPeter J Houghton

Abstract

To develop a highly reproducible model of disseminated childhood neuroblastoma in mice to allow secondary evaluation of therapeutics against microscopic disseminated disease. CB17/Icr SCID were injected i.v. with 10(3) to 5 x 10(6) human NB-1691 neuroblastoma cells. NB-1691 cells were detected by PCR for synaptophysin and tyrosine hydroxylase in peripheral blood, and bone marrow. Therapeutic studies evaluated topotecan and vincristine as single agents or in combination. Topotecan was administered i.v. daily for 5 days on two consecutive weeks. Courses were repeated every 21 days for three cycles. Vincristine (1 mg/kg) was administered i.v. every 7 days for nine consecutive weeks. Treatment started 11-21 days after tumor cell inoculation. Following injection of > or = 1 x 10(5) cells 100% of mice developed disease. Mice inoculated with 10(7) cells survived a median of 42 days. Survival time was a linear function of the cell inoculum. At autopsy, gross tumor was routinely detected in many organs in particular liver, ovaries, kidneys and adrenals. NB-1691 cells were detected by PCR in peripheral blood, and bone marrow. Immunohistochemical staining showed that lesions were strongly positive for synaptophysin, chromogranin A and neg...Continue Reading

Citations

Jul 8, 2005·Cancer Chemotherapy and Pharmacology·Carlos Rodriguez-GalindoVictor M Santana
Feb 1, 2005·Clinical & Experimental Metastasis·K Cecilia HenrikssonChristina L Chang
Aug 29, 2007·Oncogene·S K RadhakrishnanA L Gartel
Dec 22, 2006·PloS One·Karen S AboodyMary K Danks
Oct 30, 2004·International Journal of Cancer. Journal International Du Cancer·Jean-Marc JosephGilles Vassal
Feb 28, 2007·Neoplasia : an International Journal for Oncology Research·Libo ZhangSylvain Baruchel
May 18, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Li-Ling LinJiin-Haur Chuang
Jul 17, 2016·British Journal of Pharmacology·Monika WierdlPhilip M Potter
Jan 26, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Andrew M DavidoffAmit C Nathwani
Aug 12, 2014·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Manuel NistalMiguel Reyes-Múgica
Sep 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lars M WagnerMary K Danks
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Dec 3, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mario OttoRupert Handgretinger

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