PMID: 7545298Aug 29, 1995Paper

Development in one dimension: the rapid differentiation of Dictyostelium discoideum in glass capillaries

Proceedings of the National Academy of Sciences of the United States of America
J T BonnerK Y Gregg

Abstract

When Dictyostelium discoideum cells are drawn into a fine glass capillary, they rapidly begin the first steps toward the formation of prestalk and prespore zones. Some of the events occur within a minute or two, whereas others follow later. The cells in the front segment are actively motile and those in the hind segment are passive. The volumes of the segments are proportional for different-sized cell masses, and those proportions are the same as those found in normal slugs. When the cells are stained with the vital dye neutral red, the anterior zone becomes darker simultaneously with the formation of the division line. Green fluorescent protein expressed from a stalk-specific promoter is synthesized mostly in the anterior end. Later, this capillary prestalk zone shows a sharp increase in alkaline phosphatase activity, which is known to be characteristic of prestalk cells.

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F Siegert, C J Weijer
Feb 11, 1994·Science·M ChalfieD C Prasher

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Citations

Aug 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L F Jaffe
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·J T Bonner
Aug 18, 2001·The Journal of Law, Medicine & Ethics : a Journal of the American Society of Law, Medicine & Ethics·B J Gilchrist
Dec 2, 2000·Differentiation; Research in Biological Diversity·K NatarajanJ A Hadwiger
Feb 22, 2017·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Juan A Arias Del AngelMariana Benítez
May 20, 2003·Evolution & Development·John Tyler Bonner

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