Development of 3D Biofabricated Cell Laden Hydrogel Vessels and a Low-Cost Desktop Printed Perfusion Chamber for In Vitro Vessel Maturation

Macromolecular Bioscience
Thomas DistlerRainer Detsch

Abstract

The vascular system represents the key supply chain for nutrients and oxygen inside the human body. Engineered solutions to produce sophisticated alternatives for autologous or artificial vascular implants to sustainably replace diseased vascular tissue still remain a key challenge in tissue engineering. In this paper, cell-laden 3D bioplotted hydrogel vessel-like constructs made from alginate di-aldehyde (ADA) and gelatin (GEL) are presented. The aim is to increase the mechanical stability of fibroblast-laden ADA-GEL vessels, tailoring them for maturation under dynamic cell culture conditions. BaCl2 is investigated as a crosslinker for the oxidized alginate-gelatin system. Normal human dermal fibroblast (NHDF)-laden vessel constructs are optimized successfully in terms of higher stiffness by increasing ADA concentration and using BaCl2 , with no toxic effects observed on NHDF. Contrarily, BaCl2 crosslinking of ADA-GEL accelerates cell attachment, viability, and growth from 7d to 24h compared to CaCl2 . Moreover, alignment of cells in the longitudinal direction of the hydrogel vessels when extruding the cell-laden hydrogel crosslinked with Ba2+ is observed. It is possible to tune the stiffness of ADA-GEL by utilizing Ba2+ as cr...Continue Reading

References

Dec 18, 2002·Journal of Biomechanics·James H-C WangSavio L-Y Woo
Jul 9, 2005·Nature Biotechnology·Rakesh K JainDai Fukumura
May 9, 2006·Biomacromolecules·Yrr A MørchGudmund Skjåk-Braek
May 17, 2006·Journal of Long-term Effects of Medical Implants·Vladimir MironovRoger R Markwald
Dec 6, 2006·Biomedical Microdevices·Ali KhademhosseiniMilica Radisic
Mar 31, 2009·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Tomonori OieYasuhide Nakayama
Dec 19, 2009·Regenerative Medicine·Swathi Ravi, Elliot L Chaikof
Jul 14, 2010·Journal of Pediatric Surgery·Tamar L MirenskyChristopher K Breuer
Jun 4, 2011·PloS One·Justin D MihDaniel J Tschumperlin
Jun 16, 2012·Journal of Biomedical Materials Research. Part a·Yrr A MørchBerit L Strand
Nov 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Loraine L Y ChiuMilica Radisic
Jan 17, 2013·Tissue Engineering. Part C, Methods·Monica L MoyaSteven C George
Apr 2, 2013·PloS One·Chenlong ZhangJoshua M Pearce
Jan 1, 2011·Journal of Functional Biomaterials·Silke WüstSandra Hofmann
Feb 28, 2015·Advanced Healthcare Materials·Pedro F CostaCédryck Vaquette
Mar 24, 2015·International Journal of Pharmaceutics·Adam L TysonLaura C Andreae
Apr 8, 2015·International Journal of Biological Macromolecules·Bapi SarkerAldo R Boccaccini
Apr 9, 2015·Biofabrication·Tobias ZehnderRainer Detsch
Nov 27, 2015·Advanced Materials·Cristina ColosiAli Khademhosseini
Dec 3, 2015·Advanced Healthcare Materials·Anthony J MelchiorriJohn P Fisher
Dec 23, 2015·Biofabrication·Atabak Ghanizadeh TabrizWenmiao Shu
Jan 9, 2016·Biofabrication·Jürgen GrollJos Malda
Jan 26, 2016·Nature Materials·A Sydney GladmanJennifer A Lewis
Mar 1, 2016·Advanced Functional Materials·Blake N JohnsonMichael C McAlpine
Mar 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·David B KoleskyJennifer A Lewis
Apr 15, 2016·Nature Protocols·Philip J KitsonLeroy Cronin
Apr 29, 2016·Nature Methods·Steven R Caliari, Jason A Burdick
May 21, 2016·Journal of Laboratory Automation·Meghan Coakley, Darrell E Hurt
Jul 15, 2016·Stem Cell Reports·Biraja C DashYibing Qyang
Sep 24, 2016·Biofabrication·Katja HölzlAleksandr Ovsianikov
Oct 7, 2016·Nature·Elizabeth Gibney
Oct 12, 2016·Scientific Reports·Kimberly A HomanJennifer A Lewis
Nov 23, 2012·Science and Technology of Advanced Materials·Janice Kal Van TamTakao Aoyagi
Mar 8, 2017·Biofabrication·Tobias ZehnderRainer Detsch
Oct 24, 2017·Npj Regenerative Medicine·William G Chang, Laura E Niklason
Nov 16, 2017·Trends in Biotechnology·Lorenzo MoroniGiovanni Vozzi
Dec 31, 2018·Journal of Materials Science. Materials in Medicine·F RutherR Detsch
Mar 21, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bapi SarkerAldo R Boccaccini

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.