Development of (99m)Tc-radiolabeled nanosilica for targeted detection of HER2-positive breast cancer

International Journal of Nanomedicine
Paolo RainoneDavide Prosperi

Abstract

The human epidermal growth factor receptor 2 (HER2) is normally associated with a highly aggressive and infiltrating phenotype in breast cancer lesions with propensity to spread into metastases. In clinic, the detection of HER2 in primary tumors and in their metastases is currently based on invasive methods. Recently, nuclear molecular imaging techniques, including positron emission tomography and single photon emission computed tomography (SPECT), allowed the detection of HER2 lesions in vivo. We have developed a (99m)Tc-radiolabeled nanosilica system, functionalized with a trastuzumab half-chain, able to act as drug carrier and SPECT radiotracer for the identification of HER2-positive breast cancer cells. To this aim, nanoparticles functionalized or not with trastuzumab half-chain, were radiolabeled using the (99m)Tc-tricarbonyl approach and evaluated in HER2 positive and negative breast cancer models. Cell uptake experiments, combined with flow cytometry and fluorescence imaging, suggested that active targeting provides higher efficiency and selectivity in tumor detection compared to passive diffusion, indicating that our radiolabeling strategy did not affect the nanoconjugate binding efficiency. Ex vivo biodistribution of (...Continue Reading

Citations

Jan 24, 2018·Contrast Media & Molecular Imaging·Juan PellicoFernando Herranz
Jul 6, 2018·Frontiers in Chemistry·Charalampos TsoukalasPenelope Bouziotis
Nov 15, 2019·Nuclear Medicine and Molecular Imaging·Daniela Miladinova
Apr 22, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Prashant DograZhihui Wang
Jan 26, 2021·Chemical Society Reviews·Juan PellicoRafael T M de Rosales
Mar 18, 2021·International Journal of Nanomedicine·Paolo RainoneSara Belloli
Apr 4, 2021·International Journal of Molecular Sciences·Braden MillerLacey R McNally
Aug 29, 2021·Pharmaceutics·Giorgia AilunoNunzio Denora
Sep 29, 2021·Journal of Labelled Compounds & Radiopharmaceuticals·Moamen M SolimanHassan A Abd El-Rehim

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Methods Mentioned

BETA
imaging techniques
biopsy
fluorescence microscopy
electrophoretic light scattering
transmission electron microscopy
dot blot
Bio-Dot Microfiltration
flow cytometry
fluorescence-activated cell sorting
fluorescence imaging

Software Mentioned

Image Studio
Zetasizer

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