Development of a 3D printed device to support long term intestinal culture as an alternative to hyperoxic chamber methods

3D Printing in Medicine
Matheus O CostaJohn C S Harding

Abstract

Most interactions between pathogenic microorganisms and their target host occur on mucosal surfaces of internal organs such as the intestine. In vitro organ culture (IVOC) provides an unique tool for studying host-pathogen interactions in a controlled environment. However, this technique requires a complex laboratory setup and specialized apparatus. In addition, issues arise when anaerobic pathogens are exposed to the hyperoxic environment required for intestinal culture. The objective of this study was to develop an accessible 3D-printed device that would allow manipulation of the gas mixture used to supply the tissue culture media separately from the gas mixture exposed to the mucosal side of explants. Porcine colon explants from 2 pigs were prepared (n = 20) and cultured for 0h, 8h, 18h and 24h using the device. After the culture period, explants were fixed in formalin and H&E stained sections were evaluated for histological defects of the mucosa. At 8h, 66% of samples displayed no histological abnormalities, whereas samples collected at 18h and 24h displayed progressively increasing rates of superficial epithelial erosion and epithelial metaplasia. The 3D-design reported here allows investigators to setup intestinal culture...Continue Reading

References

Nov 1, 1992·Pediatric Research·D D Black, H Ellinas
Nov 28, 2002·Journal of Pediatric Gastroenterology and Nutrition·Ricardo RuedaAngel Gil
Nov 26, 2010·In Vitro Cellular & Developmental Biology. Animal·Michael K DameJames Varani
Mar 9, 2011·Cell Biology and Toxicology·Kevin J RandallJohn R Foster
Oct 22, 2016·In Vitro Cellular & Developmental Biology. Animal·Matheus O CostaJanet E Hill

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Citations

Sep 7, 2018·Current Opinion in Gastroenterology·Kenneth D SwansonEfi Kokkotou

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Software Mentioned

123D Design

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