Development of a Bacterial Biosensor for Rapid Screening of Yeast p-Coumaric Acid Production.

ACS Synthetic Biology
Solvej SiedlerHaakan N Joensson

Abstract

Transcription factor-based biosensors are used to identify producer strains, a critical bottleneck in cell factory engineering. Here, we address two challenges with this methodology: transplantation of heterologous transcriptional regulators into new hosts to generate functional biosensors and biosensing of the extracellular product concentration that accurately reflects the effective cell factory production capacity. We describe the effects of different translation initiation rates on the dynamic range of a p-coumaric acid biosensor based on the Bacillus subtilis transcriptional repressor PadR by varying its ribosomal binding site. Furthermore, we demonstrate the functionality of this p-coumaric acid biosensor in Escherichia coli and Corynebacterium glutamicum. Finally, we encapsulate yeast p-coumaric acid-producing cells with E. coli-biosensing cells in picoliter droplets and, in a microfluidic device, rapidly sort droplets containing yeast cells producing high amounts of extracellular p-coumaric acid using the fluorescent E. coli biosensor signal. As additional biosensors become available, such approaches will find broad applications for screening of an extracellular product.

References

Dec 1, 1989·Microbiological Reviews·U Kües, U Stahl
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·R HeimR Y Tsien
Aug 16, 2006·FEMS Microbiology Letters·Phillipa CashinRonan O'Toole
Aug 26, 2009·Lab on a Chip·Linas MazutisAndrew D Griffiths
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jeremy J AgrestiDavid A Weitz
Jan 19, 2012·Lab on a Chip·Tobias W HofmannChristian H J Böhm
May 5, 2012·Metabolic Engineering·Joshua K Michener, Christina D Smolke
Jun 13, 2012·Journal of Biotechnology·Jan Marienhagen, Michael Bott
Nov 28, 2012·Angewandte Chemie·Haakan N Joensson, Helene Andersson Svahn
Jan 31, 2013·Nature Biotechnology·Wenyan JiangLuciano A Marraffini
Nov 29, 2013·ACS Synthetic Biology·Solvej SiedlerMichael Bott
Dec 25, 2013·Lab on a Chip·Staffan L SjostromHelene Andersson Svahn
May 2, 2014·Nature Methods·Sriram Kosuri, George M Church
May 27, 2014·ACS Synthetic Biology·Mads T BondeHarris H Wang
Dec 3, 2014·Proceedings of the National Academy of Sciences of the United States of America·Srivatsan RamanGeorge M Church
Jan 13, 2015·Current Opinion in Biotechnology·Lothar EggelingJan Marienhagen
Jun 13, 2015·Microbial Cell Factories·Philana V van Summeren-WesenhagenJan Marienhagen
Aug 12, 2015·Proceedings of the National Academy of Sciences of the United States of America·Mingtao HuangJens Nielsen
Nov 2, 2015·Applied Microbiology and Biotechnology·Regina Mahr, Julia Frunzke
Nov 28, 2015·Applied Microbiology and Biotechnology·Nicolai KallscheuerJan Marienhagen
Nov 28, 2015·Biotechnology for Biofuels·Petter HammarHaakan N Joensson
Jan 12, 2016·Nature Methods·Mads T BondeMorten O A Sommer
May 4, 2016·ACS Synthetic Biology·Peter RugbjergMorten Otto Alexander Sommer
Oct 19, 2016·Nature Chemical Biology·Mette L SkjoedtJay D Keasling
Oct 25, 2016·Nature Chemical Biology·Hans J GeneeMorten O A Sommer

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Citations

Jan 18, 2018·Applied Microbiology and Biotechnology·Lars MilkeNicolai Kallscheuer
Jan 11, 2020·Applied Microbiology and Biotechnology·Qinghui YuGuoquan Hu
May 12, 2020·Frontiers in Bioengineering and Biotechnology·Hyewon LeeSeung-Goo Lee
Jun 11, 2020·Biomolecules·Egle ValancieneNaglis Malys
Jul 18, 2018·Nature Chemical Biology·Eric van der HelmMorten O A Sommer
Mar 7, 2020·Frontiers in Bioengineering and Biotechnology·Jia-Wei LiYun-Peng Bai
Jun 26, 2020·Frontiers in Microbiology·Carolyne Caetano GonçalvesNádia Skorupa Parachin
Oct 23, 2019·Biotechnology for Biofuels·Guadalupe Alvarez-Gonzalez, Neil Dixon
Oct 1, 2020·Synthetic Biology·Jeremy ArmettaIoana Grigoras
Aug 8, 2020·Frontiers in Microbiology·Jhonatan A Hernandez-ValdesOscar P Kuipers
Aug 25, 2020·Journal of Microbiology and Biotechnology·Shenghu ZhouJingwen Zhou
Oct 6, 2020·Metabolic Engineering·Matthew RienzoMichael D Leavell
Feb 27, 2021·Frontiers in Microbiology·Ilenne Del ValleLauren B Stadler
Jun 27, 2019·Biosensors & Bioelectronics·Niharika GuptaBansi D Malhotra
Mar 19, 2021·Critical Reviews in Biotechnology·Jianhua YangLeilei Zhu
Dec 15, 2019·Trends in Biotechnology·Emily K Bowman, Hal S Alper
Jun 19, 2021·Chemical Society Reviews·Dong YiUwe T Bornscheuer
May 18, 2021·The New Phytologist·Constantine GaragounisKalliope K Papadopoulou
Aug 10, 2021·ACS Synthetic Biology·Lion Konstantin FlachbartJan Marienhagen
Nov 7, 2019·Journal of Agricultural and Food Chemistry·Song GaoJian Chen
Aug 5, 2021·Journal of Industrial Microbiology & Biotechnology·Jennifer A Kaczmarek, Kristala L J Prather
Oct 20, 2018·ACS Synthetic Biology·Leonie BaumannMislav Oreb
Aug 29, 2019·ACS Synthetic Biology·Aditya M Kunjapur, Kristala L J Prather
Jul 30, 2019·ACS Synthetic Biology·Larry J MilletJoshua K Michener
Sep 4, 2021·Proceedings of the National Academy of Sciences of the United States of America·Emily K BowmanHal S Alper

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