Development of a bioartificial nerve graft. II. Nerve regeneration in vitro

Biotechnology Progress
Gregory E Rutkowski, Carole A Heath

Abstract

A promising alternative for the repair of peripheral nerve injuries is the bioartificial nerve graft, or BNG, comprised of a tubular conduit preseeded with Schwann cells, which are an effective substrate for enhancing nerve regeneration. The physical properties of the conduit, porosity and wall thickness, as well as the Schwann cell seeding density, were tested for their effect on axon growth using rat dorsal root ganglia. These parameters can influence the amount of nutrients and growth factors made available to the neural tissue. Results show that a greater wall thickness and lower porosities have a detrimental effect on the growth of the axons. Over a four week period, axons extended 3.2 mm for the optimum case (DeltaR = 0.82 mm, epsilon = 0.75) compared to 1.8 and 1.6 mm for a lower porosity (0.55) and a greater wall thickness (1.4 mm), respectively. A maximum in the growth rate occurs at a porosity of 75% for Schwann cell seeded conduits but not for unseeded ones. When compared to mass transfer predictions, the results suggest that, at higher porosities, more growth factors diffuse out of the conduit, while at low porosities there is competition for nutrients. Increasing the Schwann cell seeding density enhances growth but...Continue Reading

Citations

Apr 2, 2005·Der Chirurg; Zeitschrift für alle Gebiete der operativen Medizen·H-G Machens, P Mailänder
Oct 16, 2007·Annals of Biomedical Engineering·Matthew J Blewitt, Rebecca Kuntz Willits
May 24, 2012·Annals of Biomedical Engineering·Anuradha SubramanianSwaminathan Sethuraman
Jan 10, 2009·Journal of Materials Science. Materials in Medicine·Mingzhu Sun, Sandra Downes
May 1, 2012·Journal of Materials Science. Materials in Medicine·Anuradha SubramanianSwaminathan Sethuraman
Dec 4, 2003·Biomaterials·Matthew D BenderKacey G Marra
Feb 14, 2004·Biomaterials·C L A-M Carmen Lia A-M Vleggeert-LankampR T W M Ralph T W M Thomeer
Aug 5, 2011·Tissue Engineering. Part B, Reviews·Alexander R NectowDavid L Kaplan
Aug 2, 2008·Journal of Neurosurgery·Carmen L A M Vleggeert-LankampEgbert Lakke
Apr 10, 2004·Neurological Research·Jason S BelkasRajiv Midha
Jun 26, 2010·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Min HuYan Wang
Sep 25, 2007·Biomaterials·Lars DreesmannBurkhard Schlosshauer
Jul 30, 2011·Journal of Biomedical Materials Research. Part a·Yi-Cheng HuangYi-You Huang
Feb 10, 2007·Neurological Research·Renato DonzelliEnricode Divitiis
Feb 13, 2013·Journal of Tissue Engineering and Regenerative Medicine·Sung-Bum KangJames J Yoo
Dec 23, 2015·Journal of Biomedical Materials Research. Part a·Yong S LeeKathryn E Uhrich
Jul 13, 2004·Journal of Biomaterials Applications·Cheng MingyuZhang Xiufang
Feb 23, 2017·The Laryngoscope·Pei ChenTessa A Hadlock
Jan 24, 2017·IEEE Transactions on Bio-medical Engineering·Se-Jun LeeLijie Grace Zhang
Mar 9, 2018·MRS Communications·Radamés Ayala-CamineroJorge Almodovar
Sep 26, 2006·Annals of Plastic Surgery·Michael B ChenWilliam C Lineaweaver
Nov 16, 2006·Journal of Biomedical Materials Research. Part a·C L A M Vleggeert-LankampE A J F Lakke
Mar 30, 2012·Biomedical Materials·Yen-Chih Lin, Kacey G Marra
Sep 23, 2006·Journal of Cellular and Molecular Medicine·Christina AllmelingPeter M Vogt
Nov 13, 2020·Frontiers in Bioengineering and Biotechnology·Benedetta E FornasariStefania Raimondo

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