Development of a biodegradable sensor platform from gold coated zein nanophotonic films to detect peanut allergen, Ara h1, using surface enhanced raman spectroscopy

Talanta
P Gizem GezerJozef L Kokini

Abstract

Peanuts are among the most common food allergies, which may result in life-threatening reactions in certain people. For this reason, it is very important to monitor the presence of peanuts in the food system. Biosensors are an emerging way of detecting allergen proteins. In this research, we present a surface enhanced Raman spectroscopy (SERS) technique to detect the main allergen protein, Ara h1. The sensors were biodegradable and made out of a corn protein, zein. Nanophotonic structures on zein films consisted of gold coated pyramid structures. It was found that both detection and quantification was possible by using a statistical clustering technique principal component analysis (PCA). An optimization in data processing yielded the result that baseline correction and shorter data collection times were needed in order to successfully cluster data. The limit of detection was found to be 0.14 mg/ml. Furthermore, specificity of the sensor was provided by functionalizing the surface with monoclonal antibodies of Ara h1. Antibody functionalization, and Ara h1 capturing was tested and identified by also utilizing PCA analysis. As a proof-of-concept, this study showed that a biodegradable platform can be used in detection of a peanu...Continue Reading

References

Oct 22, 2005·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·M OsterballeC Bindslev-Jensen
Dec 14, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Carolina V Di AnibalItziar Ruisánchez
Jan 16, 2015·International Journal of Food Microbiology·Kristin WeidemaierMark Muldoon

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Citations

Jan 27, 2017·Annual Review of Food Science and Technology·Rohollah SadeghiJozef L Kokini
Nov 9, 2017·International Journal of Molecular Sciences·Monserrat Escamilla-GarcíaCarlos Regalado-González
Apr 20, 2019·Biosensors·Roberto PilotLaura Fabris
Sep 19, 2018·Nanomaterials·Min JiaHongyan Zhang
Jan 29, 2021·Nanomaterials·Rebeca MoldovanRadu Oprean
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Linglin FuYanbo Wang
Dec 13, 2016·Current Opinion in Biotechnology·Yun Wang, Timothy V Duncan
Mar 27, 2021·Annual Review of Food Science and Technology·Hazal Turasan, Jozef Kokini
Mar 30, 2021·Critical Reviews in Food Science and Nutrition·Fatma BoukidMassimo Castellari
Jul 3, 2021·Biosensors·Marlen PetersenXiaonan Lu
Aug 23, 2018·ACS Applied Materials & Interfaces·Victoria M SzlagChristy L Haynes

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