Development of a bsd-blasticidin selection system in Plasmodium berghei

FEBS Letters
Akira SogaShinya Fukumoto

Abstract

Plasmodium berghei is used as a rodent model for the study of malaria. However, multiple genetic manipulations are restricted by the paucity of selectable markers. The bsd-blasticidin selection system is widely used for eukaryotic cells; however, it could not previously be used for P. berghei due to toxicity to the rodent host. Here, we report the application of this selection system in P. berghei using an in vitro selection method. The desired bsd-integrated mutants are enriched by more than 90% within 2 weeks when using this system. Furthermore, the bsd marker can be used sequentially with established pyrimethamine- and puromycin-resistant markers. This system allows deeper understanding of malaria parasite biology through extensive genetic manipulation of P. berghei.

References

Dec 1, 1991·Experimental Cell Research·M IzumiF Hanaoka
Sep 1, 1996·Transactions of the Royal Society of Tropical Medicine and Hygiene·K H RieckmannM D Edstein
May 5, 1997·FEBS Letters·M OkabeY Nishimune
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·C B MamounD E Goldberg
Oct 6, 2000·Annual Review of Microbiology·T F de Koning-WardA P Waters
Oct 19, 2001·Molecular and Biochemical Parasitology·T F de Koning-WardB S Crabb
Oct 20, 2001·The Journal of Veterinary Medical Science·S FukumotoT Mikami
Jul 29, 2004·Molecular and Biochemical Parasitology·Blandine Franke-FayardChris J Janse
Nov 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Bharath BaluJohn H Adams
Nov 26, 2009·Critical Reviews in Biochemistry and Molecular Biology·Daniel N Wilson
Jul 29, 2010·Trends in Parasitology·Chris J JanseShahid M Khan
Jul 30, 2010·Trends in Parasitology·Daniel E GoldbergAndrew P Waters
Mar 20, 2015·Pathogens and Global Health·Joachim M Matz, Taco W A Kooij
Apr 17, 2015·Malaria Journal·UNKNOWN WHO Malaria Policy Advisory Committee and Secretariat

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Citations

Mar 6, 2021·Biochemical and Biophysical Research Communications·Akira SogaShinya Fukumoto

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