Development of a cancer DNA phenotype prior to tumor formation

Proceedings of the National Academy of Sciences of the United States of America
D C MalinsKarl Erik Hellström

Abstract

Using the carcinogen 3-methylcholanthrene (MCA), we demonstrate with Fourier transform-infrared spectroscopy that a cancer DNA phenotype is produced well in advance of palpable tumors. We further demonstrate that the administration of cyclophosphamide markedly inhibits the development of the cancer phenotype and concomitantly delays tumor formation. MCA, injected into the hind legs of mice, produced a variety of significant structural changes in the nucleotide bases and phosphodiester-deoxyribose backbone, as reflected in a substantial (34%) difference between the mean DNA spectra of the control and the MCA-injected mice. Strikingly, 57 days before the mean appearance of tumors, we could not distinguish the DNA structure of the histologically normal tissues of the MCA-injected mice from the DNA structure of the tumor tissues. This finding indicates the early development of a cancer phenotype. Confirmatory evidence was obtained when tissues from a group of mice injected with both MCA and cyclophosphamide did not manifest the cancer phenotype, and their mean DNA structure closely resembled that of the control mice. Accordingly, we propose that the cancer DNA phenotype, as evinced by Fourier transform-infrared spectroscopy, is a p...Continue Reading

References

Sep 14, 1978·Nature·I Hellström, K E Hellström
Oct 15, 1977·International Journal of Cancer. Journal International Du Cancer·H W KingP Brookes
Sep 1, 1992·Cancer Metastasis Reviews·J A Hickman
Feb 19, 1996·Mutation Research·L A Loeb, F C Christians
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·D C MalinsS J Gunselman
Aug 1, 1997·Nature Medicine·D C MalinsS J Gunselman
May 26, 1998·Japanese Journal of Cancer Research : Gann·K ShimokadoO Niwa
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·D C MalinsM Vinson
Mar 16, 2000·Journal of the National Cancer Institute
May 10, 2000·Clinical Pharmacokinetics·A V Boddy, S M Yule
Nov 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·D C MalinsM A Vinson
Oct 24, 2001·The Journal of Organic Chemistry·James B. SpringerSusan M. Ludeman
Nov 28, 2001·Molecular Pharmacology·P S Schwartz, D J Waxman
Jan 31, 2002·Journal of Chromatography. B, Biomedical Sciences and Applications·F Baumann, R Preiss
Apr 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Donald C MalinsKatie M Anderson
Mar 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·Donald C MalinsJerry L Spivak

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Citations

Dec 3, 2009·Journal of Immunotherapy·Pu LiuKarl Erik Hellstrom
Mar 17, 2007·Cancer Cell International·Gabriel D DakuboRyan L Parr
Jun 9, 2006·Environmental Health Perspectives·Donald C MalinsMiral Dizdaroglu
May 20, 2006·International Journal of Environmental Research and Public Health·Carolyn B HowardMarvin A Cuchens
Aug 30, 2008·International Journal of Biological Sciences·Yan-gao Man, William A Gardner
Apr 4, 2009·International Journal of Biological Sciences·Aijun LiuYan-Gao Man
Aug 18, 2010·International Journal of Biological Sciences·Yi-Hsuan HsiaoYan-gao Man
Mar 25, 2006·Future Oncology·R K Sahu, S Mordechai
Oct 11, 2007·Pharmacogenomics·David I EllisRoyston Goodacre
Dec 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Donald C MalinsKatie M Anderson
Jul 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Donald C MalinsKatie M Anderson
Sep 11, 2010·Trends in Biotechnology·Cyril Petibois, Bernard Desbat
Aug 19, 2011·The FEBS Journal·Shekhar SahaSiddhartha S Jana
Aug 29, 2006·Trends in Biotechnology·Cyril Petibois, Gérard Déléris
Oct 28, 2016·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Matthew PedersenVicki Schlegel

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