Development of a candidate reference measurement procedure for the determination of 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in human serum using isotope-dilution liquid chromatography-tandem mass spectrometry.

Analytical Chemistry
Susan S-C TaiKaren W Phinney

Abstract

Vitamin D exists in two major forms, vitamin D(3) and vitamin D(2). Vitamin D helps the body absorb calcium and promote optimal bone health. Both forms of vitamin D are metabolized to 25-hydroxyvitamin D in the body, and the levels of 25-hydroxyvitamin D(3) [25(OH)D(3)] and 25-hydroxyvitamin D(2) [25(OH)D(2)] in serum are considered the best indicators of vitamin D status. A candidate reference measurement procedure for serum 25(OH)D(3) and 25(OH)D(2) has been developed and critically evaluated. The deuterated compounds 25(OH)D(3)-d(3) and 25(OH)D(2)-d(3) are used as internal standards for 25(OH)D(3) and 25(OH)D(2), respectively. The 25(OH)D(3) and 25(OH)D(2) and their respective labeled internal standards are simultaneously extracted from serum using liquid-liquid extraction prior to reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS). Chromatographic separation was performed using a cyano (CN) column for both 25(OH)D(3) and 25(OH)D(2). Atmospheric pressure chemical ionization (APCI) in the positive ion mode and multiple reaction monitoring (MRM) were used for LC-MS/MS. The accuracy of the method was evaluated by recovery studies of measuring 25-hydroxyvitamin D [25(OH)D] in spiked samples with known 25(OH...Continue Reading

References

Jul 9, 2004·The Journal of Clinical Endocrinology and Metabolism·Bruce W Hollis
Aug 10, 2004·Annals of Clinical Biochemistry·Joseph E Zerwekh
Apr 1, 2006·Clinical Chemistry·Gary L LensmeyerMarc K Drezner
Jan 2, 2007·The Journal of Steroid Biochemistry and Molecular Biology·G D CarterJ L Berry
Dec 20, 2007·Current Opinion in Clinical Nutrition and Metabolic Care·Erin D Michos, Michal L Melamed
Jun 16, 2009·Annals of Epidemiology·Cedric F GarlandFrank C Garland

❮ Previous
Next ❯

Citations

Jul 16, 2010·Analytical and Bioanalytical Chemistry·Shujing DingDietrich A Volmer
Jan 15, 2013·Calcified Tissue International·William D Fraser, Anna M Milan
Dec 26, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Zengliu SuYusheng Zhu
Dec 7, 2011·Analytical Chemistry·Karen W PhinneyMary Frances Picciano
Sep 26, 2012·Neurology·Cynthia BalionParminder Raina
Oct 1, 2010·The Journal of Nutrition·Elizabeth A YetleyMary Frances Picciano
Mar 30, 2013·Reviews in Endocrine & Metabolic Disorders·Johannes M W van den OuwelandSilvia Bächer
Dec 11, 2013·Bioanalysis·Fabio P GomesAmitha K Hewavitharana
Dec 18, 2013·Bioanalysis·Mariëtte Theodora Ackermans, Erik Endert
Mar 27, 2015·Journal of Human Hypertension·V MajumdarR Christopher
Oct 8, 2013·Best Practice & Research. Clinical Endocrinology & Metabolism·Christopher-John Farrell, Markus Herrmann
Aug 28, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Mary BednerSusan S-C Tai
Jun 17, 2016·British Journal of Biomedical Science·Marina ParraXavier Filella
Aug 3, 2016·The British Journal of Nutrition·Sonja SoininenTimo A Lakka
Apr 5, 2017·Analytical Chemistry·Karen W PhinneyStephen P J Brooks
Dec 3, 2014·The Journal of Steroid Biochemistry and Molecular Biology·G D CarterJ L Berry
Mar 21, 2017·The Journal of Steroid Biochemistry and Molecular Biology·G D CarterE L Williams
Dec 17, 2016·The Journal of Steroid Biochemistry and Molecular Biology·N BinkleyC T Sempos
Mar 23, 2017·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·E JakabH P Bhattoa
Jan 12, 2013·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·Kim RobienJill M Hamilton-Reeves
Dec 2, 2017·Nutrients·Christine L TaylorPatsy M Brannon
Oct 2, 2015·The American Journal of Clinical Nutrition·Kurtis SarafinStephen Pj Brooks
Jul 1, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Markus HerrmannEtienne Cavalier
Sep 22, 2015·Journal of Bone Metabolism·Jun Hyung LeeAe Ja Park
Jul 28, 2018·Endocrine Connections·Federica SaponaroRiccardo Zucchi
Oct 6, 2012·The Journal of Clinical Endocrinology and Metabolism·F Granado-LorencioR Silvestre-Mardomingo
Mar 2, 2013·The Journal of Clinical Endocrinology and Metabolism·Andrew N Hoofnagle, Mara Y Roth
Sep 22, 2012·Mass Spectrometry Reviews·Rose M GathunguPaul Vouros
Jul 23, 2019·Cell Biochemistry and Function·Carl Jenkinson
Sep 21, 2013·American Journal of Clinical Pathology·Earle W HolmesKathleen M McKenna

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.