Development of a capillary electrophoresis-mass spectrometry method using polymer capillaries for metabolomic analysis of yeast

Electrophoresis
Yoshihide TanakaHitoshi Iwahashi

Abstract

Metabolomics is an emerging field in analytical biochemistry, and the development of such a method for comprehensive and quantitative analysis of organic acids, carbohydrates, and nucleotides is a necessity in the era of functional genomics. When a concentrated yeast extract was analyzed by CE-MS using a successive multiple ionic-polymer layer (SMIL)-coated capillary, the adsorption of the contaminants on the capillary wall caused severe problems such as no elution, band-broadening, and asymmetric peaks. Therefore, an analytical method for the analysis of anionic metabolites in yeast was developed by pressure-assisted CE using an inert polymer capillary made from poly(ether etherketone) (PEEK) and PTFE. We preferred to use the PEEK over the PTFE capillary in CE-MS due to the easy-to-use PEEK capillary and its high durability. The separation of anionic metabolites was successfully achieved with ammonium hydrogencarbonate/formate buffer (pH 6.0) as the electrolyte solution. The use of 2-propanol washing after every electrophoresis run not only eliminated wall-adsorption phenomena, but allowed for good repeatability to be obtained for migration times in the metabolomic analysis.

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Citations

Oct 20, 2009·Analytical and Bioanalytical Chemistry·C HuhnG W Somsen
Mar 20, 2013·Chemical Reviews·Naomi L Kuehnbaum, Philip Britz-McKibbin
Jul 14, 2010·Journal of Bioscience and Bioengineering·Daisuke Yasokawa, Hitoshi Iwahashi
Jun 12, 2009·Electrophoresis·Christian W Klampfl
Dec 25, 2008·Electrophoresis·Rawi RamautarGerhardus J de Jong
Dec 30, 2009·Electrophoresis·Eulsik OhYoung Sook Yoo
Mar 31, 2015·Electrophoresis·Elena Ortiz-VillanuevaRomà Tauler
May 21, 2019·Electrophoresis·Marlien van MeverRawi Ramautar

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