Development of a capillary gel electrophoresis method for monitoring disulfide isomer heterogeneity in IgG2 antibodies

Electrophoresis
Nathan A LacherCharles W Demarest

Abstract

A CGE method for monitoring the disulfide isomer distribution characteristic of IgG2 MAbs is presented. Disulfide heterogeneity of MAbs has been studied using various chromatographic and electrophoretic methods. Although CGE operates using a different selectivity mechanism from that of sorption chromatographic techniques, similar trends are present in the data, which allow the CGE method to be used as a complementary method for studying disulfide isomer distribution. This article focuses on the optimization of a capillary-based gel electrophoresis method that can be used to support antibody development including bioprocess optimization, antibody characterization, release, and formulation stability assessment.

References

Jan 1, 1971·The Biochemical Journal·C Milstein, B Frangione
Mar 4, 2000·The Journal of Pediatrics·M Weinberger
Jul 3, 2004·Surgery·W-C LienW-J Chen
Apr 20, 2006·Nature Reviews. Immunology·Paul J Carter
Sep 11, 2007·Journal of Pharmaceutical Sciences·Hongcheng LiuJoanne Sun
Dec 11, 2007·Nature Biotechnology·Jochen G Salfeld
Mar 15, 2008·The Journal of Biological Chemistry·Jette WypychAlain Balland
Mar 15, 2008·The Journal of Biological Chemistry·Thomas M DillonPavel V Bondarenko
Jun 14, 2008·Biochemistry·Theresa MartinezAlain Balland
Aug 21, 2008·The Journal of Biological Chemistry·Y Diana LiuGregory C Flynn
Sep 23, 2008·Electrophoresis·Richard R RustandiYang Wang
Dec 17, 2008·Current Pharmaceutical Biotechnology·Janice M Reichert
Jan 9, 2009·Electrophoresis·Xiaoyu ChenGregory C Flynn

❮ Previous
Next ❯

Citations

May 27, 2011·Journal of Biological Engineering·Mandy Ly SinPak Kin Wong
Feb 22, 2011·Journal of Pharmaceutical and Biomedical Analysis·Aline StaubSerge Rudaz
Oct 14, 2011·Electrophoresis·Tao ZhangYukui Zhang
Aug 24, 2010·Journal of Separation Science·Qian WangCharles W Demarest
Oct 15, 2013·Electrophoresis·Shuai Sherry Zhao, David D Y Chen
Nov 30, 2011·Analytica Chimica Acta·Zaifang ZhuShaorong Liu
May 28, 2013·Journal of Pharmaceutical and Biomedical Analysis·Zhiqing C ZhuBo Qiu
Jun 7, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Rabah GahoualYannis-Nicolas François
Oct 17, 2019·Analytical and Bioanalytical Chemistry·Jennifer RömerChristian Neusüß
Oct 25, 2020·Pharmaceutical Research·Bin-Bin ShenWei-Jie Fang
Jan 1, 2021·Analytical Chemistry·Cassandra L Crihfield, Lisa A Holland

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.