Development of a capillary zone electrophoresis method for dose determination in a tetravalent dengue vaccine candidate

Electrophoresis
Melissa HammRichard R Rustandi

Abstract

Dengue is known to cause morbidity and mortality worldwide and currently there is neither available specific therapeutics to treat nor a vaccine to prevent this disease. Although efforts are being made, development of a vaccine against this disease remains challenging. Hawaii Biotech Inc developed a recombinant subunit envelope protein-based vaccine against all four serotypes produced in Drosophila S2 cells which were transferred over to Merck in 2010. Each subunit of the four dengue serotypes contains the N-terminal 80% of the amino acids comprising the envelope protein (DEN-80E). A Phase 1 study using only monovalent DEN1-80E was done by Hawaii Biotech Inc and most recently, a Phase 1 clinical trial of the tetravalent DEN-80E formulation (V180) was conducted. Here, we report the development of a dose assay for the tetravalent dengue vaccine-containing subunit protein of DEN1-80E, DEN2-80E, DEN3-80E, and DEN4-80E using various separation methods such as HPLC and CE. Based on the results of the comparison, the CZE separation was chosen as the most suitable method to perform the dose assay for the tetravalent dengue vaccine.

References

Jan 23, 2004·Nature·Yorgo ModisStephen C Harrison
Sep 3, 2004·Structure·Ying ZhangMichael G Rossmann
Dec 21, 2004·Nature Reviews. Microbiology·Suchetana MukhopadhyayMichael G Rossmann
Dec 23, 2004·Journal of Virology·Yorgo ModisStephen C Harrison
Feb 22, 2011·Expert Review of Vaccines·Robert Edelman
Apr 26, 2011·Current Opinion in Immunology·Beth-Ann G Coller, David E Clements
Mar 12, 2013·Methods in Molecular Biology·Richard R RustandiMelissa Hamm
Mar 12, 2013·Methods in Molecular Biology·Richard R Rustandi
Feb 1, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Bernd MoritzMark Lies

❮ Previous
Next ❯

Citations

Feb 7, 2019·Pathogens and Disease·Stephan T Kudlacek, Stefan W Metz
May 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Binh Thanh Nguyen, Min-Jung Kang
Jan 4, 2018·Analytical Chemistry·Robert L C VoetenGovert W Somsen
Nov 6, 2021·Electrophoresis·Lars GeurinkCari Sänger-van de Griend

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.