PMID: 9188826May 9, 1997Paper

Development of a chromatographic method for the isolation and detection of hygromycin B in biological fluids

Journal of Chromatography. B, Biomedical Sciences and Applications
N F CampbellM B Medina

Abstract

An affinity chromatography method was developed for the purification of hygromycin B from biological fluids. Lysozyme and alpha-lactalbumin were immobilized on an N-hydroxysuccinimide activated agarose support. Hygromycin B solubilized in water was bound by the proteins and subsequently eluted using 10 mM sodium citrate buffer, pH 4.0. Hygromycin B was purified from swine plasma, bovine serum and bovine milk samples using a combination of ion-exchange chromatography for initial clean-up of spiked biological samples followed by affinity chromatography. Thin layer chromatographic analysis of the isolated hygromycin B revealed one band with the same R(F) value as the hygromycin B standard.

References

Apr 25, 1977·Biochemical and Biophysical Research Communications·J M Fernández-SousaR Rodriguez
Jul 1, 1992·Journal of Analytical Toxicology·J Scheurer, C M Moore
Oct 12, 1990·Journal of Chromatography·F JehlH Monteil
Apr 22, 1969·Biochimica Et Biophysica Acta·R C DaviesB M Wilson
Jan 6, 1995·Journal of Chromatography. B, Biomedical Applications·M B Medina, J J Unruh

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Citations

Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·D A Stead

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