PMID: 7520989Sep 1, 1994Paper

Development of a cloning assay with high cloning efficiency to detect induction of 6-thioguanine-resistant lymphocytes in spleen of adult mice following in vivo inhalation exposure to 1,3-butadiene

Mutation Research
A D TatesA T Natarajan

Abstract

A cloning assay with high cloning efficiency has been developed to detect spontaneous and induced 6-thioguanine-resistant T-lymphocytes (HPRT mutants) from the spleen of adult mice. The mean cloning efficiency in untreated male mice of 20-22 weeks old was 34.5 +/- 11.2% (SD) and the corresponding mutant frequency 0.7 +/- 0.8 (SD) x 10(-6). The cloning efficiencies obtained in this study are substantially higher than those reported previously by other investigators. Using this assay, it could be demonstrated that inhalation exposure of mice to 200, 500 or 1300 ppm of 1,3-butadiene for 6 h/day on 5 consecutive days caused a statistically significant induction of 6-thioguanine-resistant mutations in T-lymphocytes from spleens of adult mice exposed to 1300 ppm. The exposure to 1300 ppm resulted in a three-fold increase of the spontaneous mutant frequency. The mutant frequency after exposure to 500 ppm was higher than the control but the increase was not significant.

References

Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·T R SkopekN F Cariello
Jan 1, 1991·Environmental and Molecular Mutagenesis·D McGregorW J Caspary
Jun 1, 1989·European Journal of Immunology·J WestermannR Pabst
Jul 1, 1987·Somatic Cell and Molecular Genetics·I M JonesT L Crippen
Jan 1, 1987·The Journal of the Society of Occupational Medicine·M S Gatley, J M Davis
Jun 1, 1982·Mutation Research·H L Liber, W G Thilly

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Citations

Jun 12, 1996·Mutation Research·I D AdlerA D Tates
Oct 1, 1994·Mutation Research·D AndersonM D Waters
Jun 9, 2001·Chemico-biological Interactions·K HughesM Walker
Dec 13, 2000·Mutation Research·J T MacGregorL Müller
Feb 17, 1998·Mutation Research·F PacchierottiR Waters
Nov 6, 2003·Toxicology·Susan W P Wijnhoven, Harry van Steeg
Dec 31, 1997·Mutation Research·A AidooD A Casciano
Feb 18, 2003·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·K HughesR Beauchamp
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·S W WijnhovenH Vrieling
Aug 6, 2005·Mutation Research·Iain B LambertGeorge R Douglas
Jul 27, 2001·Environmental and Molecular Mutagenesis·H E SussmanV E Walker
Apr 18, 2008·Environmental and Molecular Mutagenesis·Irene M JonesDavid O Nelson
Sep 28, 2010·Critical Reviews in Toxicology·Richard J AlbertiniMichael L Gargas
Jan 1, 1997·Critical Reviews in Toxicology·M W HimmelsteinJ A Bond

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