Abstract
Our previous studies on outer membrane vesicles based vaccine development against shigellosis, revealed the inability of Shigella to release significant amount of vesicles naturally, during growth. Disruption of tolA, one of the genes of the Tol-Pal system of Gram negative bacterial membrane, has increased the vesicle release rate of a Shigella boydii type 4 strain to approximately 60% higher. We also noticed the vesicles, released from tolA-disrupted strain captured more OmpA protein and lipopolysaccharide, compared to the vesicles released from its wild type prototype. Six to seven weeks old BALB/c mice, immunized with 25 μg of three oral doses of the vesicles, released by tolA mutant, conferred 100% protection against lethal homologous challenge through nasal route, compared to only 60% protection after the same dose of wild type immunogen. Mice, immunized with the vesicles from tolA-mutant, manifested significant secretion of mucosal IgG and IgA. A sharp and significant response of pro-inflammatory cytokines (TNF-α, IL-6, IFN-γ) were also observed in the lung lavage of these groups of mice, within 6h post challenge; but at 24h, these inflammatory cytokines showed the sign of subsidence and the system was taken over by the r...Continue Reading
References
Mar 1, 1992·Molecular Microbiology·J C Lazzaroni, R Portalier
Jun 1, 1990·Immunology Today·C H JuneC B Thompson
Aug 15, 1970·Nature·U K Laemmli
May 26, 1995·Gene·P P Cherepanov, W Wackernagel
Jun 16, 1997·The Journal of Experimental Medicine·D F ToughJ Sprent
Jul 21, 1998·Annals of the New York Academy of Sciences·A R Coates, B Henderson
Jul 7, 1999·Vaccine·R DalsegB Haneberg
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
May 8, 2002·Molecular Microbiology·Anne WalburgerYves Corda
Feb 4, 2003·The Journal of Antimicrobial Chemotherapy·Shai AshkenaziZmira Samra
Jul 14, 2004·Research in Microbiology·Thomas HenryRoland Lloubès
Dec 20, 2005·Molecular Microbiology·Carlos BalsalobreSun Nyunt Wai
Sep 16, 2006·Journal of Leukocyte Biology·Rajwardhan YadavAnthony T Vella
Oct 14, 2006·Annals of the Rheumatic Diseases·W NiedbalaF Y Liew
Jun 15, 2007·Nature Reviews. Microbiology·Myron M LevineMarcelo B Sztein
Sep 18, 2008·Bulletin of the World Health Organization·Cynthia Boschi-PintoKenji Shibuya
Jan 2, 2009·Microbiology·G K PatersonD J Maskell
Mar 10, 2009·Gene·Shouji YamamotoHaruo Watanabe
Sep 10, 2010·Annual Review of Microbiology·Adam Kulp, Meta J Kuehn
Jan 5, 2011·Immunological Reviews·Marcela F PasettiMyron M Levine
Feb 22, 2012·The Journal of Biological Chemistry·Debasis PoreManoj K Chakrabarti
Mar 21, 2012·Critical Reviews in Immunology·Shankar Subramanian Iyer, Gehong Cheng
Jun 16, 2012·PloS One·Francesco Berlanda ScorzaChristiane Gerke
Jul 6, 2012·FEMS Immunology and Medical Microbiology·Soma MitraHemanta Koley
Jul 25, 2012·Infection and Immunity·Rebecca G AllenMichael T Bailey
May 18, 2013·Lancet·Karen L KotloffMyron M Levine
May 21, 2013·Vaccine·Soma MitraHemanta Koley
Jun 15, 2013·Vaccine·Debasis Pore, Manoj K Chakrabarti
Jul 16, 2014·The Journal of Biological Chemistry·Omar RossiChristiane Gerke
Oct 7, 2014·PloS One·Rajsekhar BhowmickManoj K Chakrabarti
Citations
Oct 26, 2016·Cellular Microbiology·Rishi D Pathirana, Maria Kaparakis-Liaskos
Jul 20, 2017·Journal of Medical Microbiology·Yadira PastorCarlos Gamazo
Jan 1, 2016·Molecular Membrane Biology·Deepak Anand, Arunima Chaudhuri
Sep 11, 2019·Cancers·Yingxuan ZhangQiong Liu
Dec 13, 2018·Diagnostic Pathology·Wei CaiHuaxi Xu
Oct 13, 2020·Critical Reviews in Microbiology·Roghayeh MohammadzadehSaman Soleimanpour
Aug 18, 2020·Applied and Environmental Microbiology·Kotaro TakakiYosuke Tashiro
Sep 29, 2019·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Linglei JiangRaymond M Schiffelers
Mar 17, 2021·Molecular Immunology·William J GilmoreMaria Kaparakis-Liaskos
Mar 30, 2021·Advanced Drug Delivery Reviews·Mina MehannyGregor Fuhrmann
May 29, 2021·Life Sciences·Abeer Ahmed Qaed AhmedGuang Yang
Dec 17, 2021·Expert Review of Vaccines·Diego PiccioliFrancesca Micoli