Development of a D genome specific marker resource for diploid and hexaploid wheat

BMC Genomics
Yi WangYong Q Gu

Abstract

Mapping and map-based cloning of genes that control agriculturally and economically important traits remain great challenges for plants with complex highly repetitive genomes such as those within the grass tribe, Triticeae. Mapping limitations in the Triticeae are primarily due to low frequencies of polymorphic gene markers and poor genetic recombination in certain genetic regions. Although the abundance of repetitive sequence may pose common problems in genome analysis and sequence assembly of large and complex genomes, they provide repeat junction markers with random and unbiased distribution throughout chromosomes. Hence, development of a high-throughput mapping technology that combine both gene-based and repeat junction-based markers is needed to generate maps that have better coverage of the entire genome. In this study, the available genomics resource of the diploid Aegilop tauschii, the D genome donor of bread wheat, were used to develop genome specific markers that can be applied for mapping in modern hexaploid wheat. A NimbleGen array containing both gene-based and repeat junction probe sequences derived from Ae. tauschii was developed and used to map the Chinese Spring nullisomic-tetrasomic lines and deletion bin line...Continue Reading

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Citations

Mar 18, 2017·Genome Génome / Conseil National De Recherches Canada·Long WangYonghong Zhou
Jan 26, 2017·Frontiers in Plant Science·Barbora BalcárkováMiroslav Valárik

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Datasets Mentioned

BETA
GSE71190

Methods Mentioned

BETA
PCR

Software Mentioned

RJPrimer
BLAST
RJPrimers
OligoCalc
Roche DEVA
Roche 454
BLASTN

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