Development of a DNA microarray to detect antimicrobial resistance genes identified in the National Center for Biotechnology Information database.

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Jonathan G FryeP J Fedorka-Cray

Abstract

To understand the mechanisms and epidemiology of antimicrobial resistance (AR), the genetic elements responsible must be identified. Due to the myriad of possible genes, a high-density genotyping technique is needed for initial screening. To achieve this, AR genes in the National Center for Biotechnology Information GenBank database were identified by their annotations and compiled into a nonredundant list of 775 genes. A DNA microarray was constructed of 70mer oligonucelotide probes designed to detect these genes encoding resistances to aminoglycosides, beta-lactams, chloramphenicols, glycopeptides, heavy metals, lincosamides, macrolides, metronidazoles, polyketides, quaternary ammonium compounds, streptogramins, sulfonamides, tetracyclines, and trimethoprims as well as resistance transfer genes. The microarray was validated with two fully sequenced control strains of Salmonella enterica: Typhimurium LT2 (sensitive) and Typhi CT18 (multidrug resistance [MDR]). All resistance genes encoded on the MDR plasmid, pHCM1, harbored by CT18 were detected in that strain, whereas no resistance genes were detected in LT2. The microarray was also tested with a variety of bacteria, including MDR Salmonella enterica serovars, Escherichia col...Continue Reading

References

Jan 1, 1995·Journal of Animal Science·S R LadelyM H Sindt
Jun 17, 2000·FEMS Microbiology Letters·N I KunongaS S Crupper
Oct 5, 2001·Clinical Microbiology Reviews·A C FluitF J Schmitz
Oct 16, 2001·Mutation Research·S PorwollikM McClelland
Apr 5, 2002·Genome Research·W James Kent
Oct 2, 2002·Current Opinion in Microbiology·Maurice Boissinot, Michel G Bergeron
Mar 26, 2003·Nucleic Acids Research·Steffen PorwollikMichael McClelland
Jun 26, 2003·Nucleic Acids Research·Henrik Bjørn NielsenSteen Knudsen
Sep 13, 2003·Journal of Applied Microbiology·D VolokhovA Rasooly
Sep 25, 2003·Antimicrobial Agents and Chemotherapy·Douglas R CallMarilyn C Roberts
Jul 9, 2004·Briefings in Functional Genomics & Proteomics·Paul Lyons
May 6, 2005·Journal of Clinical Microbiology·Vincent PerretenJoachim Frey
Jun 28, 2005·Nucleic Acids Research·Rasmus Wernersson, Henrik Bjørn Nielsen
Aug 2, 2005·The Journal of Antimicrobial Chemotherapy·Laurent MamelliJean-Michel Bolla
Aug 16, 2005·Current Opinion in Microbiology·Carol A Holland, Frederick L Kiechle
Oct 12, 2005·Archives of Medical Research·Alfonso J Alanis
Dec 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Gary J VoraDavid A Stenger
Jan 5, 2006·Applied Microbiology and Biotechnology·S Jana, J K Deb
Jan 24, 2006·International Journal of Antimicrobial Agents·Jonathan G FryePaula J Fedorka-Cray
May 26, 2006·Antimicrobial Agents and Chemotherapy·Marco CassoneGianni Pozzi
Aug 30, 2006·Methods in Enzymology·David P KreilSteven Russell
Oct 13, 2006·Molecular and Cellular Probes·Burkhard MalornyReiner Helmuth
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Marco CassoneGianni Pozzi
Apr 4, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Menggen MaShigui Liu
May 8, 2007·International Journal of Medical Microbiology : IJMM·Xiaolei YuTill T Bachmann
Sep 28, 2007·The Journal of Antimicrobial Chemotherapy·T MajtanV Majtan
Jan 11, 2008·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Katie L HopkinsE John Threlfall
Apr 1, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S MoneckeR Ehricht
May 13, 2008·Journal of Food Protection·Lori L McGowan-SpicerCharlene R Jackson
Sep 10, 2008·Antimicrobial Agents and Chemotherapy·Dave Bryson CaldwellJun Lin

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Citations

Mar 10, 2011·Foodborne Pathogens and Disease·Jonathan G FryePaula J Fedorka-Cray
Apr 5, 2011·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·LaShanda M GlennJonathan G Frye
Mar 6, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Lashanda M GlennJonathan G Frye
Jan 29, 2013·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·LaShanda M GlennJonathan G Frye
Aug 16, 2011·Applied and Environmental Microbiology·Rebecca L LindseyRichard J Meinersmann
Jan 26, 2016·Journal of Microbiological Methods·S DinarelliG Longo
Mar 20, 2010·Current Opinion in Biotechnology·Dave Boxrud
Mar 23, 2017·Frontiers in Microbiology·Janine T BosséPaul R Langford
May 31, 2017·Microarrays·Ahsan MunirSyed A Hashsham
Nov 9, 2012·Journal of Cheminformatics·Sunghwan KimStephen H Bryant
May 7, 2019·Diagnostics·Zeeshan A KhanSeungkyung Park
Jan 25, 2014·Nature Reviews. Cancer·Dominik SturmStefan M Pfister
Mar 29, 2014·Journal of Receptor and Signal Transduction Research·Wei-Hong QinHua-Sheng Liang
Jul 10, 2020·Lab on a Chip·Rathina Kumar ShanmugakaniSaurabh Mehta
Jan 1, 2013·Open Journal of Cardiovascular Surgery·Leopold Rupprecht, Christof Schmid
Jan 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hassan WaseemMuhammad Ishtiaq Ali
Apr 4, 2021·Antibiotics·Sandor KasasRonnie G Willaert

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Methods Mentioned

BETA
PCR
chip

Software Mentioned

discontinuous
OligoWiz
- Like Alignment Tool ( BLAT )
GenePix Pro
ScanArray Express
megablast
BLAST

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