Development of a dual fluorogenic and chromogenic dipeptidyl peptidase IV substrate

Bioorganic & Medicinal Chemistry Letters
Nan-Hui HoChing-Hsuan Tung

Abstract

A new far-red dual fluorogenic and chromogenic substrate, 5-glycylprolylglycylprolyl-9-di-3-sulfonyl-propylaminobenza[a]phenoxazonium perchlorate (GPGP-2SBPO), was developed for dipeptidyl peptidase IV (DPP-IV) sensing. The glycylprolylglycylprolyl tetrapeptide was chosen as the recognition sequence due to its stability under physiological conditions. In contrast, the truncated substrate, GP-2SBPO, containing only a glycylprolyl peptide, is unstable. Proteolysis of GPGP-2SBPO was assayed by monitoring the absorbance and fluorescence signals from the released fluorochrome, 2SBPO, at 625 and 670nm, respectively.

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Citations

Jun 30, 2010·Bioconjugate Chemistry·Thomas ReinerRalph Weissleder
Dec 1, 2012·Journal of the American Chemical Society·Naama Karton-LifshinDoron Shabat
Jul 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Thomas ReinerRalph Weissleder
Feb 4, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Cornelis J F Van Noorden
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Aug 25, 2018·Chemical Society Reviews·Hong-Wen LiuWeihong Tan
Mar 23, 2018·Beilstein Journal of Organic Chemistry·Futa OgawaMasaaki Omote
Jan 4, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiumei GuoHong Gao
Feb 16, 2007·Chembiochem : a European Journal of Chemical Biology·Nan-Hui HoChing-Hsuan Tung
Sep 4, 2008·Organic Letters·Jean-Alexandre RichardAnthony Romieu
Jul 23, 2008·Bioconjugate Chemistry·Jean-Alexandre RichardAnthony Romieu
May 27, 2021·Analytical Methods : Advancing Methods and Applications·Jing ZhangLi-Wei Zou
Sep 17, 2009·Bioconjugate Chemistry·Benedict Law, Ching-Hsuan Tung
Jun 3, 2011·Journal of the American Chemical Society·Naama Karton-LifshinDoron Shabat
Nov 2, 2019·Analytical Chemistry·Yuxing LinMinyong Li

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