Development of a fast and simple LC-MS/MS method for measuring the F2-isoprostanes in newborns

The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
Bruno CasettaGiuseppe Buonocore

Abstract

Quantification of F(2)-IsoPs isomer has been regarded as the "gold standard" to assess oxidative stress status in various adult and neonatal human diseases. These methods require high amounts of plasma. To develop a fast and simple LC-MS/MS method for measuring F(2)-isoprostanes in newborns. A sample of heel blood (0.4 mL) was collected in a tube containing EDTA was collected from 20 term healthy newborns. For measurements, the tandem mass spectrometer has been run in multiple reaction monitoring (MRM) with the electrospray source operating in negative ion mode, and by exploiting the transitions m/z 353.3 > 193.2 for F2-IsoPs and 357.3 > 197.2 for the internal standard d4-8-iso PGF2α. The concentration of F(2)-IsoPs (in pg/mL) in the collected cord bloods was 60.50 ± 25.04 (mean ± S.D.). No statistical difference was found between male (57.09 ± 19.69) and female (64.67 ± 31.13) concentrations. The overall efficiency of the extraction has been over 80%, while the recovery on spiked samples has been around 94% for spikes of 100 pg/mL with a C.V. of 7.7%. We developed a suitable method for large-scale studies with a reduced sample requirement as it is mandatory in neonatal age. Small samples and quick answers are very useful in Ne...Continue Reading

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Citations

Aug 8, 2015·Clinics in Perinatology·Maria Luisa TatarannoGiuseppe Buonocore
May 29, 2014·Oxidative Medicine and Cellular Longevity·Elżbieta MillerJoanna Saluk
Dec 3, 2014·Biochimica Et Biophysica Acta·Ginger L MilneL Jackson Roberts
Aug 11, 2012·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Serafina PerroneGiuseppe Buonocore
May 18, 2017·Mediators of Inflammation·Mariangela LonginiSerafina Perrone
Apr 30, 2019·Frontiers in Molecular Neuroscience·Xingping QinRenzhong Liu
Oct 6, 2020·Frontiers in Pediatrics·Caterina CovielloCarlo Dani
Jul 28, 2018·Oxidative Medicine and Cellular Longevity·Simona NegroSerafina Perrone
Feb 16, 2021·Frontiers in Pediatrics·Caterina CovielloMaria Luisa Tataranno

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