Development of a fast liquid chromatography-tandem mass spectrometry method for the determination of endocrine-disrupting compounds in waters

Analytical and Bioanalytical Chemistry
Marina Di CarroEmanuele Magi

Abstract

A fast liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS) method was developed to study five endocrine-disrupting compounds (4-n-nonylphenol, bisphenol A, estrone, 17β-estradiol and 17α-ethinylestradiol) in water. Different columns were tested; the chromatographic separation of the analytes was optimized on a Pinnacle DB biphenylic column with a water-acetonitrile gradient elution, which allowed the separation of the selected endocrine-disrupting compounds (EDCs) in less than 6 min. Quantitative analysis was performed in selected reaction monitoring (SRM) mode; two transitions were chosen for each compound, using the most abundant for quantitation. Calibration curves using bisphenol A-d (16) as internal standard were drawn, showing good correlation coefficients (0.9993-0.9998). All figures of merit of the method were satisfactory; limits of detection were in the low pg range for all analytes. The method was then applied to the determination of the analytes in real water samples: to this aim, polar organic chemical integrative samplers (POCIS) were deployed in the influent and in the effluent of a drinking water treatment plant in Liguria (Italy). The EDC level was rather low in the influent an...Continue Reading

References

May 7, 1998·Chemosphere·B Halling-SørensenS E Jørgensen
Apr 11, 2001·Fresenius' Journal of Analytical Chemistry·B K ChoiA I Gusev
Jul 12, 2002·Rapid Communications in Mass Spectrometry : RCM·Tom BenijtsAndré De Leenheer
Sep 27, 2002·Environmental Science & Technology·Thomas A TernesNinette Zulei-Seibert
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Koichi InoueHiroyuki Nakazawa
Jul 3, 2004·Environmental Toxicology and Chemistry·David A AlvarezStanley E Manahan
Dec 13, 2005·Water Research·S Rodriguez-MozazD Barceló
Jan 5, 2007·Analytical and Bioanalytical Chemistry·M FarréD Barceló
Jul 19, 2008·Journal of Chromatography. a·Ana Ballesteros-GómezDolores Pérez-Bendito
Apr 16, 2009·Mass Spectrometry Reviews·H Gallart-AyalaM T Galceran
Sep 9, 2009·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Mira PetrovicDamià Barcelo

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Citations

Sep 27, 2012·Environmental Toxicology and Chemistry·Christopher HarmanEtiënne L M Vermeirssen
Dec 3, 2014·Environmental Science & Technology·David T TanPaige J Novak
Dec 18, 2015·Dose-response : a Publication of International Hormesis Society·Jone CorralesBryan W Brooks

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