Development of a Fast SARS-CoV-2 IgG ELISA, Based on Receptor-Binding Domain, and Its Comparative Evaluation Using Temporally Segregated Samples From RT-PCR Positive Individuals.

Frontiers in Microbiology
Farha MehdiGaurav Batra

Abstract

SARS-CoV-2 antibody detection assays are crucial for gathering seroepidemiological information and monitoring the sustainability of antibody response against the virus. The SARS-CoV-2 Spike protein's receptor-binding domain (RBD) is a very specific target for anti-SARS-CoV-2 antibodies detection. Moreover, many neutralizing antibodies are mapped to this domain, linking antibody response to RBD with neutralizing potential. Detection of IgG antibodies, rather than IgM or total antibodies, against RBD is likely to play a larger role in understanding antibody-mediated protection and vaccine response. Here we describe a rapid and stable RBD-based IgG ELISA test obtained through extensive optimization of the assay components and conditions. The test showed a specificity of 99.79% (95% CI: 98.82-99.99%) in a panel of pre-pandemic samples (n = 470) from different groups, i.e., pregnancy, fever, HCV, HBV, and autoantibodies positive. Test sensitivity was evaluated using sera from SARS-CoV-2 RT-PCR positive individuals (n = 312) and found to be 53.33% (95% CI: 37.87-68.34%), 80.47% (95% CI: 72.53-86.94%), and 88.24% (95% CI: 82.05-92.88%) in panel 1 (days 0-13), panel 2 (days 14-20) and panel 3 (days 21-27), respectively. Higher sensitiv...Continue Reading

References

Jul 1, 1989·Medical Decision Making : an International Journal of the Society for Medical Decision Making·D K McClish
May 1, 1993·Journal of Clinical Epidemiology·T ByrtJ B Carlin
Mar 19, 2011·BMC Bioinformatics·Xavier RobinMarkus Müller
Jun 27, 2012·Journal of Immunological Methods·Yuhong Xiao, Stuart N Isaacs
Jan 23, 2020·Journal of Medical Virology·Yu ChenDeyin Guo
Mar 4, 2020·Nature Microbiology·UNKNOWN Coronaviridae Study Group of the International Committee on Taxonomy of Viruses
Apr 14, 2020·Annals of Internal Medicine·Matthew P ChengCedric P Yansouni
Apr 29, 2020·Clinical Chemistry·Christopher W Farnsworth, Neil W Anderson
May 1, 2020·Journal of Clinical Microbiology·Elitza S TheelKamran Kadkhoda
May 14, 2020·Nature Medicine·Fatima AmanatFlorian Krammer
Jun 9, 2020·Immunity·Nicolas VabretUNKNOWN Sinai Immunology Review Project
Jun 20, 2020·Nature Medicine·Quan-Xin LongAi-Long Huang
Jun 28, 2020·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Thomas NicolHélène Le Guillou-Guillemette
Jul 3, 2020·The Indian Journal of Medical Research·Gajanan SapkalUNKNOWN $COVID Support Team: Pawar S., Patshute S., Salve V., Natarajan V., Marwah V., Malhotra B., Jain A., Sathe P., More R., Khed
Jul 25, 2020·The New England Journal of Medicine·F Javier IbarrondoOtto O Yang
Jul 28, 2020·The Journal of Applied Laboratory Medicine·C S LauT C Aw
Aug 4, 2020·BioRxiv : the Preprint Server for Biology·Jason T LadnerJohn A Altin
Aug 21, 2020·Emerging Microbes & Infections·Sophie van TolChantal Reusken
Aug 29, 2020·Nature Biotechnology·Jeffrey D WhitmanAlexander Marson
Sep 2, 2020·The New England Journal of Medicine·Daniel F GudbjartssonKari Stefansson
Sep 11, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·C S LauT C Aw
Sep 15, 2020·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Julien MarletCatherine Gaudy-Graffin
Oct 2, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Álvaro A Faccini-MartínezVeronica Contreras
Oct 9, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Tahmina ShirinFirdausi Qadri

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
transfection
gel filtration
Assay
ELISAs

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.