PMID: 2500487Jun 21, 1989Paper

Development of a fluorescent immunodot assay for Bacillus cereus enterotoxin

Journal of Immunological Methods
S G Jackson

Abstract

A fluorescent immunodot assay has been developed for rapid, specific detection of B. cereus enterotoxin. None of the other Bacillus species tested showed cross-reactivity in the assay with antiserum to purified B. cereus enterotoxin. The assay can detect greater than or equal to 50 ng of purified enterotoxin. Using this assay system, enterotoxin was found to be produced by 25 of 25 foodborne disease-related isolates and 22 of 25 isolates not related to foodborne disease (isolates from routine surveillance foods). Because of the apparent widespread ability of isolates to produce enterotoxin the assay may have potential as a rapid identification procedure for B. cereus. The substrate gel system described may have wider application in other immunoassay systems using a membrane solid phase.

References

Aug 1, 1975·Canadian Journal of Microbiology·W M Spira, J M Goepfert
Apr 1, 1978·The Journal of Hygiene·A C Ghosh
Oct 1, 1976·Journal of Clinical Pathology·P C Turnbull
Dec 1, 1985·The Journal of Hygiene·P C Turnbull, J M Kramer

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Citations

Aug 1, 1995·Letters in Applied Microbiology·S G JacksonS Kasatiya
Jan 11, 2000·Clinical Microbiology Reviews·M A Ghannoum
Aug 17, 2011·Journal of Thyroid Research·Taimur SaleemQamar Masood
Oct 1, 1993·Clinical Microbiology Reviews·F A Drobniewski
Apr 1, 1993·Journal of Clinical Microbiology·S G Jackson

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