Development of a fluorescent indicator for the bioimaging of nitric oxide

Biological & Pharmaceutical Bulletin
H KojimaT Nagano

Abstract

Nitric oxide (NO) has been reported to play various roles as a signal transmitter. However, detailed functions of NO have yet to be clarified. We have developed a fluorescent indicator for NO imaging in living cells. The N-nitrosation of newly designed and synthesized 4-((3-amino-2-naphthyl)aminomethyl)benzoic acid (DAN-1) by NO yielded the highly fluorescent triazole-form. The membrane permeable ester derivative of DAN-1 (DAN-1 EE) was applied to the imaging of NO produced in activated rat aortic smooth muscle cells. After DAN-1 EE has been loaded into cells, the ester bond is hydrolyzed by intracellular esterase, yielding original DAN-1 with less permeability. The fluorescence intensity of the cells loaded with DAN-1 EE increased according to NO production. The imaging method with fluorescent indicators will be significant for the functional clarification of NO in vivo.

Citations

Sep 8, 2011·Inorganic Chemistry·Michael D PluthStephen J Lippard
Oct 22, 2003·Biological Procedures Online·Thomas R. RäthelVerena M. Dirsch
Feb 16, 2006·Journal of Fluorescence·Ana GomesJosé L F C Lima
May 5, 2011·Angewandte Chemie·Yoshinori KawazoeMotonari Uesugi
Jun 3, 2014·Chemical Communications : Chem Comm·Xin LvWei Guo
Jun 22, 2006·Chembiochem : a European Journal of Chemical Biology·Mi Hee LimStephen J Lippard
Jun 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qing WangXianshun Zeng
Apr 11, 2002·Chemical Reviews·Tetsuo Nagano, Tetsuhiko Yoshimura
Apr 20, 2021·ACS Sensors·Bethany AlmeidaJames B Delehanty

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