Development of a fraction collection approach in capillary electrophoresis SELEX for aptamer selection

The Analyst
Zhaofeng LuoLiyun Zhang

Abstract

Aptamers have attracted much attention due to their ability to bind to target molecules with high affinity and specificity. The development of an approach capable of efficiently generating aptamers through systematic evolution of ligands by exponential enrichment (SELEX) is particularly challenging. Herein, a fraction collection approach in capillary electrophoresis SELEX (FCE-SELEX) for the partition of a bound DNA-target complex is developed. By integrating fraction collection with a facile oil seal method for avoiding contamination while amplifying the bound DNA-target complex, in a single round of selection, a streptavidin-binding aptamer (SBA) has been generated. The affinity of aptamer SBA-36 for streptavidin (SA) is determined as 30.8 nM by surface plasmon resonance (SPR). Selectivity and biotin competition experiments demonstrate that the SBA-36 aptamer selected by FCE-SELEX is as efficient as those from other methods. Based on the ability of fraction collection in partition and collection of the aptamer-target complex from the original DNA library, FCE-SELEX can be a universal tool for the development of aptamers.

References

Oct 25, 1995·Nucleic Acids Research·K P Williams, D P Bartel
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·I Wong, T M Lohman
Sep 20, 2000·Accounts of Chemical Research·M FamulokM Blind
Sep 10, 2002·Nature Biotechnology·Joshua A BittkerDavid R Liu
Sep 23, 2003·Nucleic Acids Research·Frédéric PileurChristian Cazenave
Jan 8, 2004·Journal of the American Chemical Society·Shaun D Mendonsa, Michael T Bowser
Apr 23, 2004·Current Opinion in Structural Biology·Gourisankar GhoshTom Huxford
Sep 15, 2004·Analytical Chemistry·Shaun D Mendonsa, Michael T Bowser
Mar 3, 2005·Journal of the American Chemical Society·Maxim BerezovskiSergey N Krylov
Jun 30, 2005·Journal of the American Chemical Society·Shaun D Mendonsa, Michael T Bowser
Jul 30, 2005·Analytical and Bioanalytical Chemistry·R StoltenburgB Strehlitz
Aug 11, 2005·Journal of the American Chemical Society·Andrei DrabovichSergey N Krylov
Nov 30, 2005·Analytical and Bioanalytical Chemistry·Glen HybargerJames P Chambers
Nov 30, 2005·Electrophoresis·Christian Schou, Niels H H Heegaard
Feb 2, 2006·Journal of the American Chemical Society·Maxim BerezovskiSergey N Krylov
Jun 23, 2006·Nature Methods·Richard WilliamsAndrew D Griffiths
Aug 8, 2007·Biomolecular Engineering·Yan YangZhiren Zhang
Feb 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xinhui LouH Tom Soh
Apr 9, 2009·Acta Biochimica Et Biophysica Sinica·Chenglong WangHongmei Ding
Nov 30, 2010·Analytical Biochemistry·Tatjana SchützeJörn Glökler
Aug 28, 2014·Talanta·Bonnie Jaskowski HugeNorman J Dovichi

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Citations

Jul 3, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hongguang Sun, Youli Zu
Mar 13, 2016·Nucleic Acids Research·Maureen McKeagueChristina D Smolke
Jun 9, 2016·Scientific Reports·Jee-Woong ParkThomas Laurell
Jan 31, 2018·Journal of Industrial Microbiology & Biotechnology·Shuobo ShiHuimin Zhao
Sep 17, 2018·World Journal of Microbiology & Biotechnology·Lijun WangYanbin Li
Jan 31, 2018·Analytical and Bioanalytical Chemistry·Audrey RicFrançois Couderc
Jul 26, 2019·World Journal of Gastroenterology : WJG·Guo-Qing ZhangYong-Xiang Zhao
Sep 16, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xixia LiuYaoyao Hou
Nov 3, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shingo Saito
Mar 5, 2021·Biomedical Chromatography : BMC·Xin Guo, Guan-Hua Chen
May 1, 2021·Nanomaterials·Olubunmi O AyodeleTetyana Ignatova
May 7, 2019·Analytical Chemistry·František ForetYann Astier

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