Development of a functional thyroid model based on an organoid culture system

Biochemical and Biophysical Research Communications
Yoshiyuki SaitoOsamu Nagano

Abstract

The low turnover rate of thyroid follicular cells and the lack of a long-term thyroid cell culture system have hampered studies of thyroid carcinogenesis. We have now established a thyroid organoid culture system that supports thyroid cell proliferation in vitro. The established mouse thyroid organoids performed thyroid functions including thyroglobulin synthesis, iodide uptake, and the production and release of thyroid hormone. Furthermore, transplantation of the organoids into recipient mice resulted in the formation of normal thyroid-like tissue capable of iodide uptake and thyroglobulin production in vivo. Finally, forced expression of oncogenic NRAS (NRASQ61R) in thyroid organoids established from p53 knockout mice and transplantation of the manipulated organoids into mouse recipients generated a model of poorly differentiated thyroid cancer. Our findings suggest that this newly developed thyroid organoid culture system is a potential research tool for the study of thyroid physiology and pathology including thyroid cancer.

Citations

Oct 31, 2018·Thyroid : Official Journal of the American Thyroid Association·Jun PanYi-Jun Wu
Sep 17, 2018·Journal of Hematology & Oncology·Hanxiao XuKongming Wu
Apr 27, 2019·Reviews in Endocrine & Metabolic Disorders·Ebtesam A Al-Suhaimi, Khulood Al-Khater
Aug 7, 2019·Disease Models & Mechanisms·Madeline A Lancaster, Meritxell Huch
Nov 29, 2020·International Journal of Urology : Official Journal of the Japanese Urological Association·Manabu KatoTakahiro Inoue
Feb 2, 2021·The Journal of Clinical Endocrinology and Metabolism·Dong ChenYongsheng Zhao
Nov 23, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Allison LewisAnne Grapin-Botton
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Aug 14, 2021·Acta Pharmaceutica Sinica. B·Yuanjin ZhangXin Wang
Aug 21, 2021·Frontiers in Cell and Developmental Biology·Meng-Meng ZhangKe-Wei Jiang
Nov 3, 2020·Cell Regeneration·Yaqi LiGuoqiang Hua
Dec 18, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jelte van der VaartHans Clevers

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