Development of a gas chromatography/ion trap mass spectrometry based method for the quantification of polybrominated diphenyl ethers and polychlorinated biphenyls in adipose tissue

Rapid Communications in Mass Spectrometry : RCM
C NaertC Van Peteghem

Abstract

A combined gas chromatographic mass spectrometric (GC/MS/MS) method for the determination of seven polybrominated diphenyl ethers (PBDEs) and seven marker polychlorinated biphenyls (PCBs) in adipose tissue has been developed. Adipose tissue was melted and filtered through anhydrous sodium sulphate to obtain pure fat. Clean-up was performed using a glass column containing acidified silica, deactivated alumina and anhydrous sodium sulphate. Polybrominated biphenyl (PBB) 155 and Mirex were added as internal standards for PBDEs and PCBs, respectively. Injection standards, PBB 103 and PCB 143, for PBDEs and PCBs, respectively, were added before analysis with GC/MS/MS. The developed GC/MS/MS method has the advantage of being more selective than single MS methods because matrix effects are largely eliminated. Validation of this method was conducted according to Commission Decision 2002/657/EC. Decision limits for PBDEs and PCBs ranged from 0.06-0.15 ng g(-1) and from 0.35-1.22 ng g(-1), respectively. Detection capabilities were all between 0.23-0.55 ng g(-1) for PBDEs and between 0.98-2.29 ng g(-1) for PCBs. Precision, recovery, bias and selectivity were tested, with satisfactory results.

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Citations

Jan 16, 2008·Analytical and Bioanalytical Chemistry·Rosa Martínez OcañaPatricia Plaza Bolaños
Jan 5, 2006·Archives of Environmental Contamination and Toxicology·C NaertC Van Peteghem
Sep 21, 2012·Journal of the American Society for Mass Spectrometry·Virgínia C FernandesCristina Delerue-Matos
Jul 27, 2005·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Kazuhiko AkutsuShinjiro Hori
Apr 11, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ying LiJin-Ming Lin
Jul 31, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lucia Liguori, Hans-René Bjørsvik
Feb 28, 2009·Journal of Chromatography. a·V YusàM de la Guardia
Sep 2, 2009·Mass Spectrometry Reviews·Dongli Wang, Qing X Li

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