PMID: 8941770Jun 1, 1996Paper

Development of a gene transfer system in Treponema denticola by electroporation

Oral Microbiology and Immunology
H Li, H K Kuramitsu

Abstract

Treponema denticola is strongly implicated in the etiology of periodontal diseases. However, genetic transformation of this organism has not been reported. We now demonstrate a gene transfer system in T. denticola by electroporation using a broad host plasmid pKT210 as a shuttle vector. Plasmid extraction, Southern blot hybridization as well as the polymerase chain reaction indicated the presence of the plasmid in T. denticola transformants. The restriction patterns of plasmid pKT210 rescued from the T. denticola transformants in Escherichea coli suggested that some of the rescued plasmids were identical to the original pKT210, but some of them had been modified. This transformation system could be a potentially useful tool for genetic manipulation of oral spirochetes.

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Citations

Mar 12, 2002·FEMS Microbiology Letters·Si Young LeeJ Christopher Fenno
Jun 8, 2001·Trends in Microbiology·F C CabelloE Y Dobrikova
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Dec 20, 2015·Applied and Environmental Microbiology·Kurni Kurniyati, Chunhao Li
Apr 19, 2015·Applied and Environmental Microbiology·Yuebin LiHui Wu
Jul 15, 2015·Applied and Environmental Microbiology·Valentina GodovikovaJ Christopher Fenno
Jan 22, 2015·Journal of Bacteriology·Jiang BianChunhao Li
Jan 11, 2020·Frontiers in Cellular and Infection Microbiology·Hong Min NgEric C Reynolds
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Jul 7, 2021·PLoS Pathogens·Emily RomeisLorenzo Giacani

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