PMID: 8595858Jan 15, 1996Paper

Development of a genus specific primer set for detection of Leishmania parasites by polymerase chain reaction

FEMS Microbiology Letters
R BhattacharyyaH K Majumder

Abstract

We have compared the sequences of a major class of kinetoplast DNA (kDNA) minicircle (pLURkE3) of Leishmania strain UR6 with other minicircle sequences from different Leishmania species. Alignment of these sequences allowed the selection of a pair of oligonucleotides suitable as primers in polymerase chain reaction (PCR) which is specific for Leishmania parasites. PCR with this genus-specific primer set is capable of detecting 1 femtogram of kDNA. These primers have been tested with kDNAs from both old world and new world Leishmania species. The results indicate that the primers may be suitable for detection of any kind of leishmaniasis.

Citations

Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Gerlind AndersCharles L Greenblatt
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Samit AdhyaSantu Bandyopadhyay
Jul 14, 2007·The Journal of Parasitology·Germano A FerreiraFrederico G C Abath
Sep 16, 2015·Journal of the European Academy of Dermatology and Venereology : JEADV·C MitteldorfW Kempf
Feb 6, 2017·Molecular Aspects of Medicine·Mohammad AkhoundiDenis Sereno
Jun 16, 2017·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Madhumita Manna, Supriya Khanra
Jul 3, 1998·Journal of Clinical Microbiology·E HarrisN Agabian

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