Development of a Graphene Oxide-Incorporated Polydimethylsiloxane Membrane with Hexagonal Micropillars

International Journal of Molecular Sciences
Yi-Ying LinChien-Ying Wang

Abstract

Several efforts have been made on the development of bioscaffolds including the polydimethylsiloxane (PDMS) elastomer for supporting cell growth into stable sheets. However, PDMS has several disadvantages, such as intrinsic surface hydrophobicity and mechanical strength. Herein, we generated a novel PDMS-based biomimetic membrane by sequential modifications of the PMDS elastomer with graphene oxide (GO) and addition of a hexagonal micropillar structure at the bottom of the biomembrane. GO was initially homogenously mixed with pure PDMS and then was further coated onto the upper surface of the resultant PDMS. The elastic modulus and hydrophilicity were significantly improved by such modifications. In addition, the development of hexagonal micropillars with smaller diameters largely improved the ion permeability and increased the motion resistance. We further cultured retinal pigment epithelial (RPE) cells on the surface of this modified PDMS biomembrane and assayed its biocompatibility. Remarkably, the GO incorporation and coating exhibited beneficial effect on the cell growth and the new formation of tight junctions in RPE cells. Taken together, this GO-modified PDMS scaffold with polyhexagonal micropillars may be utilized as a...Continue Reading

References

Jan 14, 2000·Electrophoresis·J C McDonaldG M Whitesides
Oct 14, 2004·Journal of Biomedical Materials Research. Part a·George K ToworfePaul Ducheyne
Oct 23, 2004·Science·K S NovoselovA A Firsov
Dec 15, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Jessamine Ng LeeGeorge M Whitesides
Oct 6, 2007·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Sandra ZelleWolf Petersen
Nov 6, 2007·Current Opinion in Biotechnology·Jeffrey M Karp, Robert Langer
Nov 14, 2008·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·B P Chan, K W Leong
Oct 6, 2009·Osteoarthritis and Cartilage·J E J BekkersW J A Dhert
Dec 8, 2009·The British Journal of Ophthalmology·Heather A J ThomsonAndrew J Lotery
Mar 10, 2011·Nanomedicine·Liangzhu Feng, Zhuang Liu
Mar 19, 2011·The British Journal of Ophthalmology·Susanne Binder
Dec 1, 1998·Analytical Chemistry·D C DuffyG M Whitesides
Mar 3, 2012·Medical Engineering & Physics·Alessandro RussoMaurilio Marcacci
Apr 20, 2013·Stem Cells Translational Medicine·David E BuchholzDennis O Clegg
Apr 25, 2013·Journal of Biomedical Materials Research. Part a·Jangho KimJong Hoon Chung
May 21, 2013·Journal of Biomaterials Science. Polymer Edition·Eileen PedrazaCherie L Stabler
Aug 21, 2013·Acta Biomaterialia·Jingquan LiuDusan Losic
Oct 29, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sumit GoenkaShilpa Sant
Feb 20, 2014·Investigative Ophthalmology & Visual Science·Kevin J McHughMagali Saint-Geniez
Apr 20, 2014·Science·Kostas Kostarelos, Kostya S Novoselov
Oct 27, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Brian D HoltStefanie A Sydlik
Jan 17, 2017·International Journal of Biological Macromolecules·S SaravananN Selvamurugan
Aug 11, 2017·Journal of Tissue Engineering·Corina E White, Ronke M Olabisi

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Citations

Oct 20, 2018·International Journal of Molecular Sciences·Jing LiQi Sun

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Methods Mentioned

BETA
atomic force microscopy
AFM
PCR
infrared spectroscopy
dissection

Software Mentioned

GraphPad Prism5

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