PMID: 11339298May 8, 2001Paper

Development of a high-performance liquid chromatographic method for the quantification of chlorpyrifos, pyridostigmine bromide, N,N-diethyl-m-toluamide and their metabolites in rat plasma and urine

Journal of Chromatography. B, Biomedical Sciences and Applications
A W Abu-Qare, M B Abou-Donia

Abstract

A method was developed for the separation and quantification of the insecticide chlorpyrifos (O,O-diethyl-O[3,5,6-trichloro-2-pyridinyl] phosphorothioate), its metabolites chlorpyrifos-oxon (O,O-diethyl-O[3,5,6-trichloro-2-pyridinyl] phosphate) and TCP (3,5,6-trichloro-2-pyridinol), the anti-nerve agent drug pyridostigmine bromide (PB; 3-dimethylaminocarbonyloxy-N-methyl pyridinium bromide), its metabolite N-methyl-3-hydroxypyridinium bromide, the insect repellent DEET (N,N-diethyl-m-toluamide), and its metabolites m-toluamide and m-toluic acid in rat plasma and urine. The method is based on using solid-phase extraction and high-performance liquid chromatography (HPLC) with reversed-phase C18 column, and gradient UV detection ranging between 210 and 280 nm. The compounds were separated using a gradient of 1-85% acetonitrile in water (pH 3.20) at a flow-rate ranging between 1 and 1.7 ml/min over a period of 15 min. The retention times ranged from 5.4 to 13.2 min. The limits of detection ranged between 20 and 150 ng/ml, while the limits of quantitation were between 150 and 200 ng/ml. Average percentage recovery of five spiked plasma samples was 80.2+/-7.9, 74.9+/-8.5, 81.7+/-6.9, 73.1+/-7.8, 74.3+/-8.3, 80.8+/-6.6, 81.6+/-7.3 and...Continue Reading

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Citations

Oct 29, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shane R NeedhamWilliam D Korte
Oct 18, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ronald E BaynesJim E Riviere
Dec 28, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ramisetti Nageswara RaoSyed Naseeruddin Alvi
Apr 2, 2013·Journal of Pharmaceutical and Biomedical Analysis·Praveen U SanganalmathBannur M Mohan
Aug 8, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Füsun Okçu PelitF Nil Ertaş
Oct 11, 2015·Analytical Biochemistry·Wirongrong WhangsukSuvit Loprasert
Dec 21, 2004·Environment International·Yongsong CaoGuoqing Sheng
Sep 3, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xu WangShiwen Xu
Jul 5, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sreeneeranj KasichayanulaXiaochen Gu

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