Development of a high throughput screening method to test flavour-forming capabilities of anaerobic micro-organisms

Journal of Applied Microbiology
Bart A SmitGerrit Smit

Abstract

Development of a fast, automated and reliable screening method for screening of large collections of bacterial strains with minimal handling time. The method is based on the injection of a small headspace sample (100 microl) from culture vials (2 ml) in 96-well format directly into the mass spectrometry (MS). A special sample tray has been developed for liquid media, and anaerobically grown cultures. In principle, all volatile components can be measured, but a representative mass fragment has to be obtained in the MS. Representative masses for 3-methylbutanal, 2-methylpropanal and benzaldehyde are 58, 72 and 105, respectively. In 1 day over 1500 samples could be analysed and the coefficient of variation for the response was <5%. Screening of 72 strains belonging to the genus Lactococcus in quadruple on the production of the key-flavour compound 3-methylbutanal illustrated the effectiveness of the method. Furthermore, knowledge of the biochemistry and physiology of 3-methylbutanal formation was used to optimize the composition of the growth medium to enhance 3-methylbutanal production, and thereby improve the screening. A commonly used method to control flavour formation in fermented food products is the selection of bacterial s...Continue Reading

References

Oct 1, 1996·Antonie van Leeuwenhoek·E R KunjiW N Konings
Jul 11, 2000·Journal of Agricultural and Food Chemistry·P Perpète, S Collin
Oct 27, 2001·Journal of Biomolecular Screening·P LaveryA J Pope
Apr 2, 2002·Analytical Chemistry·Christophe PérèsJean-Louis Berdagué
Sep 18, 2002·Analytical Biochemistry·Arthur J L CooperSusan M Hutson
Jan 7, 2003·Applied and Environmental Microbiology·Renske LandeweertEric Smit
Jan 22, 2003·Applied Microbiology and Biotechnology·M ArroyoM Pilar Castillón

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Citations

Nov 19, 2008·Applied Microbiology and Biotechnology·Bart A SmitGerrit Smit
Jun 7, 2005·FEMS Microbiology Reviews·Gerrit SmitWim J M Engels
Jan 17, 2013·Critical Reviews in Food Science and Nutrition·María Del Carmen Martínez-CuestaTeresa Requena
Jun 10, 2008·Applied and Environmental Microbiology·Mengjin LiuRoland J Siezen
Jul 12, 2011·Applied and Environmental Microbiology·Frank A M de BokDouwe Molenaar
Mar 7, 2006·Current Opinion in Biotechnology·Johan E T van Hylckama VliegRoland J Siezen
Jan 1, 2012·Computational and Structural Biotechnology Journal·Markus Herrgård, Gianni Panagiotou
Mar 4, 2014·Annual Review of Food Science and Technology·E J Smid, M Kleerebezem
Nov 20, 2009·Journal of Dairy Science·H BachmannJ E T van Hylckama Vlieg
Aug 24, 2017·FEMS Microbiology Reviews·Jan KokOscar P Kuipers

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