Development of a Highly Sensitive Device for Counting the Number of Disease-Specific Exosomes in Human Sera

Clinical Chemistry
Yasuaki KabeHiroshi Handa

Abstract

Although circulating exosomes in blood play crucial roles in cancer development and progression, difficulties in quantifying exosomes hamper their application for reliable clinical testing. By combining the properties of nanobeads with optical disc technology, we have developed a novel device named the ExoCounter to determine the exact number of exosomes in the sera of patients with various types of cancer. In this system, individual exosomes were captured in the groove of an optical disc coated with antibodies against exosome surface antigens. The captured exosomes were labeled with antibody-conjugated magnetic nanobeads, and the number of the labeled exosomes was counted with an optical disc drive. We showed that the ExoCounter could detect specific exosomes derived from cells or human serum without any enrichment procedures. The detection sensitivity and linearity with this system were higher than those with conventional detection methods such as ELISA or flow cytometry. In addition to the ubiquitous exosome markers CD9 and CD63, the cancer-related antigens CD147, carcinoembryonic antigen, and human epidermal growth factor receptor 2 (HER2) were also used to quantify cancer cell line-derived exosomes. Furthermore, analyses o...Continue Reading

References

Mar 1, 1996·The Journal of Experimental Medicine·G RaposoH J Geuze
Oct 26, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shengming DaiXuetao Cao
Apr 27, 2007·Neoplasia : an International Journal for Oncology Research·Danilo MillimaggiVincenza Dolo
Jan 30, 2008·Current Protocols in Cell Biology·Clotilde ThéryAled Clayton
Feb 27, 2009·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Satoshi SakamotoHiroshi Handa
Jun 29, 2012·Journal of Translational Medicine·Annette M MarleauRichard H Tullis
Sep 7, 2013·Journal of Extracellular Vesicles·Chris GardinerIan L Sargent
Sep 7, 2013·Journal of Extracellular Vesicles·Kenneth W WitwerFred Hochberg
Feb 19, 2014·Nature Reviews. Rheumatology·Edit I BuzasSteffen Gay
Apr 9, 2014·Nature Communications·Yusuke YoshiokaTakahiro Ochiya
Apr 23, 2014·Nature Biotechnology·Hyungsoon ImHakho Lee
Oct 8, 2014·Annual Review of Cell and Developmental Biology·Marina ColomboClotilde Théry
Oct 29, 2014·Journal of Visualized Experiments : JoVE·Sybren L N MaasMarike L D Broekman
Nov 14, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lawrence RajendranXandra O Breakefield
Dec 17, 2014·Journal of Extracellular Vesicles·Frank A W CoumansRienk Nieuwland
Mar 5, 2015·Journal of Extracellular Vesicles·Kristine R JakobsenMalene M Jørgensen
Jun 25, 2015·Nature·Clotilde Théry
Jan 7, 2016·Frontiers in Cellular Neuroscience·Dora Brites, Adelaide Fernandes
Feb 11, 2016·International Journal of Molecular Sciences·Hina KalraSuresh Mathivanan
Apr 14, 2016·Scientific Reports·Mark A RiderDavid G Meckes
Jul 29, 2016·Scientific Reports·Abu Ali Ibn SinaMatt Trau
Feb 13, 2017·Journal of Epidemiology·Akiko NagaiMichiaki Kubo

❮ Previous
Next ❯

Citations

Mar 30, 2019·Translational Psychiatry·Saumeh SaeediGustavo Turecki
Jan 11, 2019·Analytical and Bioanalytical Chemistry·Yasuaki KabeHiroshi Handa
Nov 13, 2020·Frontiers in Oncology·Carolina HerreroLaura Muinelo-Romay
Mar 9, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Guohao LiYao Wu
Nov 12, 2020·Current Drug Metabolism·Yin-Xue WangYi-Qing Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.