Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA

BMC Research Notes
Carolina SenraEdward Oliveira

Abstract

We developed a laboratorial platform to release a commercial platform used in the PCR-ELISA for the molecular diagnosis of schistosomiasis mansoni. On following, PCR-ELISA platform laboratorial was evaluated in 206 feces samples collected of individual living in a Brazilian low endemicity area. The PCR-ELISA laboratorial platform indicated a prevalence rate of 25.2%, which was higher than the Kato-Katz technique (18.4%) and lower than the commercial platform (30.1%). Considering Kato-Katz technique as the reference, there were 97.4% and 91.1% of relative sensitivity and specificity rates, respectively. The laboratorial platform presented good precision, performance diagnostic, and can be used in replacement to the commercial platform for diagnosis of schistosomiasis by PCR-ELISA.

References

Jan 1, 1991·Molecular and Biochemical Parasitology·J HamburgerR Deresiewicz
Jul 24, 2002·The American Journal of Tropical Medicine and Hygiene·Luís André PontesAna Rabello
Feb 20, 2007·Memórias do Instituto Oswaldo Cruz·Ana Lisa do Vale GomesFrederico G C Abath
Apr 28, 2010·PLoS Neglected Tropical Diseases·Luciana Inácia GomesAna Rabello
Jan 10, 2014·Memórias do Instituto Oswaldo Cruz·Teiliane Rodrigues CarneiroFernando Schemelzer Moraes Bezerra
Sep 9, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Maysa Ahmad Eraky, Nagwa Shaban Mohamed Aly

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Citations

Apr 4, 2019·Memórias do Instituto Oswaldo Cruz·Vanessa Silva-MoraesPaulo Marcos Zech Coelho
Sep 3, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Hong-Mei LiXiao-Nong Zhou

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Datasets Mentioned

BETA
M61098

Methods Mentioned

BETA
PCR
PCR-ELISA

Software Mentioned

GraphPad Prism
OpenEpi

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