Development of a LC-MS/MS Method for the Simultaneous Detection of Tricarboxylic Acid Cycle Intermediates in a Range of Biological Matrices

Journal of Analytical Methods in Chemistry
Omar Al KadhiShikha Saha

Abstract

It is now well-established that perturbations in the tricarboxylic acid (TCA) cycle play an important role in the metabolic transformation occurring in cancer including that of the prostate. A method for simultaneous qualitative and quantitative analysis of TCA cycle intermediates in body fluids, tissues, and cultured cell lines of human origin was developed using a common C18 reversed-phase column by LC-MS/MS technique. This LC-MS/MS method for profiling TCA cycle intermediates offers significant advantages including simple and fast preparation of a wide range of human biological samples. The analytical method was validated according to the guideline of the Royal Society of Chemistry Analytical Methods Committee. The limits of detection were below 60 nM for most of the TCA intermediates with the exception of lactic and fumaric acids. The calibration curves of all TCA analytes showed linearity with correlation coefficients r(2) > 0.9998. Recoveries were >95% for all TCA analytes. This method was established taking into consideration problems and limitations of existing techniques. We envisage that its application to different biological matrices will facilitate deeper understanding of the metabolic changes in the TCA cycle from...Continue Reading

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Citations

Nov 6, 2018·The Journal of Biological Chemistry·Brian D FinkWilliam I Sivitz
Dec 13, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Samantha GokhalePing Xie
Jul 31, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Brian D FinkWilliam I Sivitz
Jul 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tharsini SivapalanShikha Saha
May 8, 2020·The Journal of Biological Chemistry·Zheng XingElizabeth J Tran
Sep 30, 2020·Proceedings of the National Academy of Sciences of the United States of America·Joel D W TohChristopher J Chang
Jan 23, 2021·Sports Medicine - Open·Maria João NunesLuís Cobra Branco
Feb 1, 2021·The Journal of Biological Chemistry·Zheng XingElizabeth J Tran
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Uyory ChoeLiangli Lucy Yu
May 29, 2021·Current Opinion in Biotechnology·Tomasz K BednarskiJamey D Young

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BETA
Nuclear Magnetic Resonance

Software Mentioned

MassHunter Quantitative

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