PMID: 9647843Jul 2, 1998Paper

Development of a lipase fermentation process that uses a recombinant Pseudomonas alcaligenes strain

Applied and Environmental Microbiology
G GerritseW J Quax

Abstract

Pseudomonas alcaligenes M-1 secretes an alkaline lipase, which has excellent characteristics for the removal of fatty stains under modern washing conditions. A fed-batch fermentation process based on the secretion of the alkaline lipase from P. alcaligenes was developed. Due to the inability of P. alcaligenes to grow on glucose, citric acid and soybean oil were applied as substrates in the batch phase and feed phase, respectively. The gene encoding the high-alkaline lipase from P. alcaligenes was isolated and characterized. Amplification of lipase gene copies in P. alcaligenes with the aid of low- and high-copy-number plasmids resulted in an increase of lipase expression that was apparently colinear with the gene copy number. It was found that overexpression of the lipase helper gene, lipB, produced a stimulating effect in strains with high copy numbers (> 20) of the lipase structural gene, lipA. In strains with lipA on a low-copy-number vector, the lipB gene did not show any effect, suggesting that LipB is required in a low ratio to LipA only. During scaling up of the fermentation process to 100 m3, severe losses in lipase productivity were observed. Simulations have identified an increased level of dissolved carbon dioxide as...Continue Reading

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Dec 1, 1992·Applied and Environmental Microbiology·L G FrenkenC T Verrips
Jul 1, 1992·Journal of General Microbiology·S WohlfarthU K Winkler
Sep 1, 1991·Journal of General Microbiology·E J GilbertC W Jones
Aug 1, 1989·The Journal of Applied Bacteriology·N M Dixon, D B Kell
Oct 11, 1988·Nucleic Acids Research·S E West, B H Iglewski
Dec 1, 1986·Journal of Bacteriology·W StuerU K Winkler
Jun 1, 1988·Journal of Bacteriology·N M Calvin, P C Hanawalt
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·V HaringM Bagdasarian
Dec 1, 1994·Molecular & General Genetics : MGG·J L AamandD J McConnell
Mar 1, 1995·Journal of Bacteriology·F IharaY Yamada
Aug 1, 1993·Molecular Microbiology·L G FrenkenC T Verrips
Jan 10, 1989·Biotechnology and Bioengineering·Y K Lee, C L Choo

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Citations

Nov 14, 2006·Applied and Environmental Microbiology·Christophe Dubuis, Dieter Haas
Jan 15, 2008·Applied and Environmental Microbiology·Joanna KrzeslakWim J Quax
Nov 15, 2011·Applied Biochemistry and Biotechnology·Zheng-Yu ShuJian-Zhong Huang
Nov 4, 2008·Applied and Environmental Microbiology·Ahmed SallamAlexander Steinbüchel
Aug 28, 2020·Microbial Cell Factories·Prem ChandraPankaj Kumar Arora
Nov 24, 2012·FEMS Microbiology Letters·Genki AkanumaMorio Ishizuka
Jul 31, 2012·Applied Biochemistry and Biotechnology·Panagiota-Yiolanda StergiouEmmanuel M Papamichael
Mar 30, 2013·Bioprocess and Biosystems Engineering·M N I SalehminY Chisti
Dec 1, 1999·Bioorganic & Medicinal Chemistry·C Schmidt-Dannert
Oct 11, 2003·Biotechnology Advances·R SharmaU C Banerjee
Mar 28, 2003·Biochimica Et Biophysica Acta·Qinhong MaMilton H Saier

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