Development of a liquid chromatography-tandem mass spectrometry method using capillary liquid chromatography and nanoelectrospray ionization-quadrupole time-of-flight hybrid mass spectrometer for the detection of milk allergens

Journal of Agricultural and Food Chemistry
Dorcas WeberBen Lau

Abstract

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of the tryptic digest of a cleaned-up food matrix extract was used for the detection of milk allergens. The emphasis of this study was on casein, which is the most abundant milk protein and is also considered the most allergenic. A sample cleanup method was developed using an ion exchange column and centriprep device. Cookies spiked with milk powder from 0 to 1250 ppm were extracted, cleaned up, and either digested directly by trypsin or further cleaned up by gel electrophoresis before digestion. The peptide mixture was analyzed on a capillary LC-quadrupole time-of-flight system. Two marker peptides from alphaS1-casein were identified and used for prescreening. The MS/MS data from the mass spectrometry system were processed with Masslynx v4.0 and submitted for database search using either ProteinLynx Global Server or Mascot for protein identification. The LC-MS/MS method, using casein enzyme-linked immunosorbent assay as a reference, was tested on the cookie matrix and was extended to other sample matrices. There were good agreements between the two. This LC-MS/MS method provides a valuable confirmatory method for the presence of casein. It also allows the simul...Continue Reading

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Citations

May 29, 2007·Analytical and Bioanalytical Chemistry·Arjon J van Hengel
Mar 25, 2009·Analytical and Bioanalytical Chemistry·Patricia Schubert-UllrichSabine Baumgartner
Jul 29, 2009·Analytical and Bioanalytical Chemistry·Dorcas WeberSamuel Benrejeb Godefroy
Jul 21, 2011·Analytical and Bioanalytical Chemistry·Rudolf KrskaDon Forsyth
Mar 8, 2011·Journal of Agricultural and Food Chemistry·Barbara SimonatoGabriella Pasini
Jun 8, 2012·Journal of Agricultural and Food Chemistry·G Asher Newsome, Peter F Scholl
Jan 18, 2013·Journal of Agricultural and Food Chemistry·Tatiana CucuBruno De Meulenaer
Jun 23, 2009·Analytical and Bioanalytical Chemistry·Stéphanie KirschEdwin De Pauw
Jan 19, 2016·Food Chemistry·Cristina LambertiGiuffrida Maria Gabriella
Apr 8, 2015·Critical Reviews in Food Science and Nutrition·M PradoJ Barros-Velázquez
Mar 18, 2015·Critical Reviews in Food Science and Nutrition·Rita C AlvesCristina Delerue-Matos
Sep 3, 2016·Journal of Agricultural and Food Chemistry·Tingting GuoJian Kong
Dec 1, 2006·Journal of Mass Spectrometry : JMS
Jul 12, 2007·Journal of Separation Science·Javier Hernández-BorgesSalvatore Fanali
Oct 31, 2009·Journal of Separation Science·María Asensio-RamosSalvatore Fanali
Jul 17, 2018·Applied Biochemistry and Biotechnology·Kun WanTadashi Hatanaka
Jan 4, 2017·Journal of Dairy Science·Katherine O IvensSteve L Taylor
Jul 21, 2017·NPJ Systems Biology and Applications·Derek Croote, Stephen R Quake
Sep 1, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Thomas HolzhauserSimon D Flanagan
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May 16, 2014·Journal of Proteome Research·Martina KoeberlAndreas L Lopata
Jul 9, 2011·Journal of Chromatography. a·Gianluca PicarielloPasquale Ferranti

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