PMID: 375075Feb 1, 1979Paper

Development of a liquid-holding technique for the study of DNA-repair in human diploid fibroblasts

Mutation Research
J W Simons

Abstract

Liquid-holding conditions can be obtained for human diploid skin fibroblasts by keeping confluent cultures stationary over periods of 7 days or longer by means of conditioned medium. Under this condition recovery of radiation damage induced by ultraviolet light or X-rays is observed as an increase in cloning efficiency. The amount of recovery when expressed in a dose-modifying-factor appears higher than in bacteria and yeast. The repair-deficient human cell strains XP25Ro and XP7Be (xeroderma pigmentosum from complementation groups A and D respectively) exhibit less but still discernible recovery after UV-irradiation and the same was observed for AT5Bi (ataxia telangiectasia) after X-irradiation. Experiments on mutation induction indicated that the repair which takes place during liquid holding of UV-irradiated XP7Be cells reduces the mutant frequency considerably while after liquid holding of UV-irradiated wild-type cells the same or lower mutant frequencies were found for the lower exposures and the same or higher mutant frequencies for the higher exposures.

Citations

Jul 1, 1986·Mutation Research·S A Leadon, P C Hanawalt
Sep 1, 1987·Mutation Research·R T JohnsonA R Collins
Aug 1, 1981·Quarterly Reviews of Biophysics·R MeneghiniR I Schumacher
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L H ThompsonL E Dillehay
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J N HowellJ J McCormick
Jul 21, 2005·Molecular Biology of the Cell·Kirk J McManus, Michael J Hendzel
Aug 20, 2008·Molecular and Cellular Biology·Lei LiRoseline Godbout
Oct 1, 1981·Chemico-biological Interactions·M F PeraJ J Roberts
Feb 18, 2014·International Journal of Radiation Biology·Sudhir ChandnaSiamak Haghdoost
May 1, 1986·Photochemistry and Photobiology·M Protić-Sabljić, K H Kraemer
Apr 1, 1994·Photochemistry and Photobiology·S M de LeeuwA A Schothorst
Apr 1, 1982·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·G L Chan, J B Little
Jan 1, 1983·Environmental Mutagenesis·W J CoppingerE D Thompson
Apr 1, 1981·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·R CoxJ B Little
Dec 1, 1983·Physics in Medicine and Biology·K H Chadwick, H P Leenhouts

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.