Development of a Listeria monocytogenes EGDe partial proteome reference map and comparison with the protein profiles of food isolates.

Applied and Environmental Microbiology
Manilduth RamnathAnne Gravesen

Abstract

A partially annotated proteome reference map of the food pathogen Listeria monocytogenes was developed for exponentially growing cells under standardized, optimal conditions by using the sequenced strain EGDe (serotype 1/2a) as a model organism. The map was developed by using a reproducible total protein extraction and two-dimensional (2-D) polyacrylamide gel electrophoresis analysis procedure, and it contained 33 identified proteins representing the four main protein functional classes. In order to facilitate analysis of membrane proteins, a protein compartmentalization procedure was assessed. The method used provided partial fractionation of membrane and cytosolic proteins. The total protein 2-D profiles of three serotype 1/2a strains and one serotype 1/2b strain isolated from food were compared to the L. monocytogenes EGDe proteome. An average of 13% of the major protein spots in the food strain proteomes were not matched in the strain EGDe proteome. The variation was greater for the less intense spots, and on average 28% of these spots were not matched. Two of the proteins identified in L. monocytogenes EGDe were missing in one or more of the food isolates. These two proteins were proteins involved in the main glycolytic pa...Continue Reading

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Citations

Dec 22, 2004·Journal of Applied Microbiology·L NilssonA Gravesen
Nov 26, 2008·Applied and Environmental Microbiology·Janet R DonaldsonMark L Lawrence
Jul 9, 2004·Applied and Environmental Microbiology·Suleyman YildirimSophia Kathariou
May 28, 2005·Canadian Journal of Microbiology·M A HeffordM L Kalmokoff
Jun 8, 2006·Journal of Biotechnology·Torsten HainTrinad Chakraborty
Jan 20, 2006·Critical Reviews in Biotechnology·Anu Kalia, R P Gupta
Aug 5, 2016·PloS One·Marcelo MendonçaJosé Antonio G Aleixo

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