Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine

Scientific Reports
Lihua LuDik-Lung Ma

Abstract

A panel of six luminescent iridium(III) complexes were synthesized and evaluated for their ability to act as G-quadruplex-selective probes. The novel iridium(III) complex 1 was found to be highly selective for G-quadruplex DNA, and was employed for the construction of a label-free G-quadruplex-based adenosine detection assay in aqueous solution. Two different detection strategies were investigated for adenosine detection, and the results showed that initial addition of adenosine to the adenosine aptamer gave superior results. The assay exhibited a linear response for adenosine in the concentration range of 5 to 120 μM (R(2) = 0.992), and the limit of detection for adenosine was 5 μM. Moreover, this assay was highly selective for adenosine over other nucleosides, and exhibited potential use for biological sample analysis.

References

Jan 17, 1995·Biochemistry·D E Huizenga, J W Szostak
Nov 10, 2004·Journal of Pharmaceutical and Biomedical Analysis·Lan-Fang HuangBen-Mei Cheng
Apr 6, 2007·Chembiochem : a European Journal of Chemical Biology·Weian ZhaoYingfu Li
Apr 29, 2008·Chemical Communications : Chem Comm·Mengxiao YuChunhui Huang
Dec 17, 2008·Chemical Communications : Chem Comm·Na LuZhaoxiang Deng
Feb 5, 2009·Nucleic Acids Research·Jaroslav KyprMichaela Vorlícková
May 21, 2009·Inorganic Chemistry·Mi Hee LimJacqueline K Barton
Jun 30, 2009·Talanta·Xiluan YanJianzhong Lu
Nov 17, 2009·Journal of Medicinal Chemistry·Haijia YuXiaogang Qu
Apr 22, 2010·Analytical Chemistry·Po-Jung Jimmy Huang, Juewen Liu
Jun 1, 2010·Analytica Chimica Acta·Chenhu SunRuqin Yu
Sep 3, 2010·The Journal of Physical Chemistry. a·Shih-Hsiang WuI-Chia Chen
Oct 16, 2010·Biosensors & Bioelectronics·Sujuan YeWei Cao
Jun 21, 2011·Journal of the American Chemical Society·Chunyan LiFuyou Li
Sep 7, 2011·Journal of the American Chemical Society·Jinliang LiuFuyou Li
Apr 4, 2012·ACS Applied Materials & Interfaces·Youssef HelwaJuewen Liu
Apr 26, 2012·Chemical Communications : Chem Comm·Yen-Hsiu Lin, Wei-Lung Tseng
Jan 26, 2013·Chemical Society Reviews·Dik-Lung MaChung-Hang Leung
Apr 6, 2013·Chemical Communications : Chem Comm·Ka-Ho LeungDik-Lung Ma
Jul 10, 2014·Analytical Chemistry·Ashley E Ross, B Jill Venton
Feb 27, 2015·Chemical Communications : Chem Comm·Achikanath C Bhasikuttan, Jyotirmayee Mohanty

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Citations

May 5, 2017·Chemistry, an Asian Journal·Dik-Lung MaChung-Hang Leung
Jul 8, 2017·Scientific Reports·Xin ZhangDihua Shangguan

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Methods Mentioned

BETA
fluorescence resonance
biosensors
fluorescence resonance energy transfer

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