Abstract
Resistance to aminoglycoside antibiotics is now common in pathogenic bacteria, making treatment of infections difficult. The rapid spread of resistance is mainly related to the dissemination of genes encoding aminoglycoside-modifying enzymes (AMEs). Staphylococci and enterococci are opportunistic human pathogens capable of causing a wide range of infections. Isolates from clinical cases are often found to be resistant to aminoglycosides. The aim of the present study was to develop a bead-based xTAG assay for the simultaneous detection of five prevalent aminoglycoside resistance genes in staphylococci and enterococci, including aac(6')-Ie-aph(2″)-Ia, aph(3')-IIIa, ant(4')-Ia, ant(9)-Ia, and ant(6)-Ia. The limit of detection ranged from 10 to 1000 copies/μL of input purified plasmid DNA. Twenty-two bacterial isolates from clinical samples were examined using the newly developed xTAG assay and also by conventional PCR to determine the relative performance of each. The results obtained by xTAG assay showed higher detection rates and accuracy for AME genes than conventional PCR. It indicated that the xTAG-multiplex PCR method is a high-throughput tool for rapid identification of AME genes.
References
Sep 1, 1991·Antimicrobial Agents and Chemotherapy·D J LeBlancJ M Inamine
Nov 1, 1990·Antimicrobial Agents and Chemotherapy·H OunissiP Courvalin
Jan 1, 1985·Molecular & General Genetics : MGG·E Murphy
May 1, 1997·Antimicrobial Agents and Chemotherapy·A DerbiseN El Solh
Sep 19, 2000·Antimicrobial Agents and Chemotherapy·S J KaoJ W Chow
Aug 30, 2001·Journal of Clinical Microbiology·T IdaM Inoue
Mar 26, 2003·Antimicrobial Agents and Chemotherapy·Sergei B VakulenkoJoseph W Chow
Oct 6, 2005·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mohammed Mahbub AlamNaoki Watanabe
Feb 17, 2009·Methods in Molecular Biology
Sep 14, 2010·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Maria S Ramirez, Marcelo E Tolmasky
Nov 6, 2013·Analytical and Bioanalytical Chemistry·Elena PonzoniLaura Morello
Mar 25, 2014·BMC Infectious Diseases·Shamshul AnsariMoti Lal Chapagain
Aug 26, 2014·TheScientificWorldJournal·Sara SoheiliRukman Awang Hamat
Dec 2, 2014·Nature Reviews. Microbiology·Jessica M A BlairLaura J V Piddock
Mar 15, 2015·International Journal of Environmental Research and Public Health·Wanxiang LiHuoyang Lv
Apr 22, 2016·Jundishapur Journal of Microbiology·Fateh Rahimi
Oct 19, 2017·Bioscience of Microbiota, Food and Health·Yuji Ohashi, Tomohiko Fujisawa
Nov 15, 2017·The Kaohsiung Journal of Medical Sciences·Azar Dokht KhosraviEffat Abbasi Montazeri