Development of a massively parallel sequencing assay for investigating sequence polymorphisms of 15 short tandem repeats in a Chinese Northern Han population

Electrophoresis
Qing-Xia ZhangJiangwei Yan

Abstract

Massively parallel sequencing (MPS) has been used in forensic genetics in recent years owing to several advantages, e.g. MPS can provide precise descriptions of the repeat allele structure and variation in the repeat-flanking regions, increasing the discriminating power among loci and individuals. However, it cannot be fully utilized unless sufficient population data are available for all loci. Thus, there is a pressing need to perform population studies providing a basis for the introduction of MPS into forensic practice. Here, we constructed a multiplex PCR system with fusion primers for one-directional PCR for MPS of 15 commonly used forensic autosomal STRs and amelogenin. Samples from 554 unrelated Chinese Northern Han individuals were typed using this MPS assay. In total, 313 alleles obtained by MPS for all 15 STRs were observed, and the corresponding allele frequencies ranged between 0.0009 and 0.5162. Of all 15 loci, the number of alleles identified for 12 loci increased compared to capillary electrophoresis approaches, and for the following six loci more than double the number of alleles was found: D2S1338, D5S818, D21S11, D13S317, vWA, and D3S1358. Forensic parameters were calculated based on length and sequence-based ...Continue Reading

References

Feb 19, 2011·Nature Reviews. Genetics·Manfred Kayser, Peter de Knijff
Dec 6, 2011·Methods in Molecular Biology·Robyn ThompsonBruce McCord
Jun 19, 2013·Forensic Science International. Genetics·David H WarshauerBruce Budowle
Dec 3, 2014·Forensic Science International. Genetics·Walther ParsonJodi A Irwin
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Feb 24, 2015·Forensic Science International. Genetics·Claus Børsting, Niels Morling
Aug 13, 2015·Forensic Science International. Genetics·Bingbing XieJiangwei Yan
Dec 25, 2015·Forensic Science International. Genetics·Katherine Butler GettingsPeter M Vallone
Apr 16, 2016·Forensic Science International. Genetics·Fei GuoXianhua Jiang

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Citations

Jan 2, 2021·Forensic Science International : Synergy·John M Butler, Sheila Willis
Nov 30, 2018·Analytical Chemistry·Bruce R McCordGeorge Duncan

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